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An ultrasensitive probe-free electrochemical immunosensor for gibberellins employing polydopamine-antibody nanoparticles modified electrode

文献类型： 外文期刊

作者： You, Yang 1 ; Luo, Bin 1 ; Wang, Cheng 1 ; Dong, Hongtu 1 ; Wang, Xiaodong 1 ; Hou, Peichen 1 ; Sun, Lijun 3 ; Li, Aixue 1 ;

作者机构： 1.Beijing Acad Agr & Forestry Sci, Res Ctr Intelligent Equipment, Beijing 100097, Peoples R China

2.Hebei Univ Engn, Coll Landscape & Ecol Engn, Handan 056038, Peoples R China

3.Nantong Univ, Sch Life Sci, 9 Seyuan Rd, Nantong 226019, Jiangsu, Peoples R China

关键词： Gibberellins; Immunosensor; Carboxylated graphene oxide; Carboxylated multi -walled carbon nanotubes; Polydopamine nanoparticles

期刊名称：BIOELECTROCHEMISTRY （ 影响因子：5.0； 五年影响因子：4.9 ）

ISSN： 1567-5394

年卷期： 2023 年 150 卷

页码：

收录情况： SCI

摘要： Gibberellins (GA3) is an ubiquitous plant hormone, which plays a regulatory role in different growth stages of plants, so it is of great significance to develop a sensitive quantitative analysis method for GA3. In this study, carboxylated graphene oxide-carboxylated multi-walled carbon nanotubes-Fc (GO-MWNT-Fc) composite ma-terial and PDANPs-antibody (PDANPs-Ab) were sequentially modified to screen-printed electrodes (SPEs), and an ultrasensitive probe-free immunosensor for GA3 was developed. Fc was applied to generate electrochemical signals. GO-COOH and MWNT-COOH can increase the catalytic ability of the sensor and bind the PDANPs-Ab nanoparticles. PDANPs nanomaterial were synthetized by a facile self-polymerization and used to bind with antibody, so as to increase the antibody loading of the sensor. The as-prepared immunosensor has the widest detection range (100 aM-1 mM) and lowest detection limit (17.4 aM) for GA3 up to date. To our knowledge, it is the first electrochemical immunosensor for GA3. By changing the GA3 antibody to ABA antibody, a sensitive and selective immunosensor for ABA was also fabricated. This immunosensor platform is simple, sensitive, and low cost. It opens broad prospect in on-site applications for biosensors in detecting of various biomolecules in pre-cision agriculture.

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