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Characterization of a novel cry8 gene specific to Melolonthidae pests: Holotrichia oblita and Holotrichia parallela

文献类型： 外文期刊

作者： Shu, Changlong 1 ; Yan, Guixin 1 ; Wang, Rongyan 2 ; Zhang, Jie 1 ; Feng, Shuliang 2 ; Huang, Dafang 3 ; Song, Fuping 1 ;

作者机构： 1.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China

2.Hebei Acad Agr & Forestry Sci, Inst Plant Protect, Beijing 071000, Peoples R China

3.Chinese Acad Agr Sci, Biotechnol Res Inst, Beijing 100081, Peoples R China

关键词： Bacillus thuringiensis;Cry8Ga;Holotrichia oblita;Holotrichia parallela;PCR-RFLP

期刊名称：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY （ 影响因子：4.813； 五年影响因子：4.697 ）

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收录情况： SCI

摘要： A new polymerase chain reaction-restriction fragment length polymorphism method for the identification of cry8-type genes from Bacillus thuringiensis has been established by designing a pair of new universal primers. By this method, a novel gene, cry8Ga1, encoding a polypeptide of 1,157 amino acids with a deduced molecular mass of 131.2 kDa was identified and cloned from B. thuringiensis HBF-18. Recombinant B. thuringiensis strain HD8G, harboring cry8Ga1, has insecticidal activity against larvae of Melolonthidae pests: Holotrichia oblita and Holotrichia parallela. This is the first report of a Cry toxin that has insecticidal activity to Melolonthidae pest H. oblita.

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