Overexpression of leucoanthocyanidin reductase or anthocyanidin reductase elevates tannins content and confers cassava resistance to two-spotted spider mite
文献类型： 外文期刊
作者： Chen, Qing 1 ; Liang, Xiao 1 ; Wu, Chunling 1 ; Liu, Ying 1 ; Liu, Xiaoqiang 1 ; Zhao, Huiping 1 ; Li, Kaimian 3 ; Chen, Songbi 3 ; Wang, Haiyan 4 ; Han, Zhiling 1 ; Wu, Mufeng 1 ; Yao, Xiaowen 1 ; Shui, Jun 1 ; Qiao, Yang 1 ; Zhan, Xue 1 ; Zhang, Yao 1 ;
作者机构： 1.Chinese Acad Trop Agr Sci, Environm & Plant Protect Inst, Key Lab Integrated Pest Management Trop Crops, Minist Agr & Rural Affairs, Haikou, Hainan, Peoples R China
2.Chinese Acad Trop Agr Sci, Sanya Res Acad, Hainan Key Lab Biosafety Monitoring & Mol Breeding, Sanya, Hainan, Peoples R China
3.Chinese Acad Trop Agr Sci, Trop Crops Genet Resources Inst, Haikou, Peoples R China
4.Chinese Acad Trop, Inst Trop Biosci & Biotechnol, Haikou, Peoples R China
关键词： cassava (Manihot esculenta Crantz); two-spotted spider mite (Tetranychus urticae Koch); leucoanthocyanidin reductase; anthocyanidin reductase; tannins (condensed); resistance mechanism
期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：6.627； 五年影响因子：7.255 ）
ISSN： 1664-462X
年卷期： 2022 年 13 卷
页码：
收录情况： SCI
摘要： The two-spotted spider mite (TSSM) is a destructive cassava pest. Intensive demonstration of resistance mechanism greatly facilitates the creation of TSSM-resistant cassava germplasm. Gene to metabolite network plays a crucial role in modulating plant resistance, but little is known about the genes and related metabolites which are responsible for cassava resistance to TSSM. Here, a highly resistant (HR) and a highly susceptible (HS) cassava cultivar were used, integrative and comparative transcriptomic and metabolomic analyses between these two cultivars after TSSM infestation revealed that several genes and metabolites were closely related and significantly different in abundance. In particular, the expression of leucoanthocyanidin reductase (LAR) and anthocyanidin reductase (ANR) genes showed a high positive correlation with most of the metabolites in the tannin biosynthesis pathway. Furthermore, transgenic cassava lines overexpressing either of the genes elevated tannin concentrations and conferred cassava resistance to TSSM. Additionally, different forms of tannins possessed distinct bioactivity on TSSM, of which total condensed tannins (LC50 = 375.68 mg/l) showed maximum lethal effects followed by procyanidin B1 (LC50 = 3537.10 mg/l). This study accurately targets LAR, ANR and specific tannin compounds as critical genes and metabolites in shaping cassava resistance to TSSM, which could be considered as biomarkers for evaluation and creation of pest-resistant cassava germplasm.
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