文献类型： 外文期刊
作者： Tan, Jihong 1 ; Lin, Lili 1 ; Luo, Haiyan 3 ; Zhou, Shengjun 2 ; Zhu, Yuqiang 2 ; Wang, Xin 2 ; Miao, Li 1 ; Wang, Huasen 1 ; Zhang, Peng 2 ;
作者机构： 1.Zhejiang Agr & Forestry Univ, Coll Hort Sci,Minist Agr & Rural Affairs, Collaborat Innovat Ctr Efficient & Green Prod Agr, Key Lab Qual & Safety Control Subtrop Fruits & Ve, Hangzhou 311300, Peoples R China
2.Zhejiang Acad Agr Sci, Hangzhou 310021, Peoples R China
3.Hangzhou Linan Dist Agr & Rural Bur, Hangzhou 311300, Peoples R China
关键词： cucumber; genetic transformation; Agrobacterium tumefaciens; transgenic plants; positive selection system; biotechnology; plant regeneration
期刊名称：APPLIED SCIENCES-BASEL （ 影响因子：2.838； 五年影响因子：2.921 ）
ISSN：
年卷期： 2022 年 12 卷 14 期
页码：
收录情况： SCI
摘要： Cucumber (Cucumis sativus L.), belonging to the gourd family (Cucurbitaceae), is one of the major vegetable crops in China. Conventional genetic breeding methods are ineffective for improving the tolerance of cucumber to various environmental stresses, diseases, and pests in the short term, but bio-engineering technologies can be applied to cucumber breeding to produce new cultivars with high yield and quality. Regeneration and genetic transformation systems are key technologies in modern cucumber breeding. Compared with regeneration systems, genetic transformation systems are not yet fully effective, and the low efficiency of genetic transformation is a bottleneck in cucumber cultivation. Here, we systematically review the key factors influencing the regeneration and genetic transformation of cucumber plants, including the selection of genotype, source of explants and forms of exogenous hormones added to the medium, the methods of transgene introduction and co-cultivation, and selection methods. In addition, we also focus on recent advances in the study of molecular mechanisms underlying important agronomic traits using genetic transformation technology, such as fruit length, fruit warts, and floral development. This review provides reference information for future research on improvements in cucumber varieties.
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