Molecular cloning and expression of Chimonanthus praecox farnesyl pyrophosphate synthase gene and its possible involvement in the biosynthesis of floral volatile sesquiterpenoids
文献类型： 外文期刊
作者： Xiang, Lin 1 ; Zhao, Kaige 1 ; Chen, Longqing 1 ;
作者机构： 1.Huazhong Agr Univ, Key Lab Hort Plant Biol, Minist Educ, Coll Hort & Forestry Sci, Wuhan 430070, Peoples R China
2.Zhejiang Acad Agr Sci, Inst Hort, Hangzhou 310021, Zhejiang, Peoples R China
关键词： Farnesyl pyrophosphate synthase;Heterologous expression;Sesquiterpenoid biosynthesis;Wintersweet
期刊名称：PLANT PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：4.27； 五年影响因子：4.816 ）
ISSN：
年卷期：
页码：
收录情况： SCI
摘要： Farnesyl pyrophosphate (FPP) synthase catalyzes the biosynthesis of FPP, which is the precursors of sesquiterpenoids such as floral scent volatiles, from isopentenyl pyrophosphate (IPP) and dimethylallyl pyrophosphate (DMAPP). cDNA encoding wintersweet (Chimonanthus praecox L.) FPP synthase was isolated by the RT-PCR and RACE methods. The deduced amino acid sequence showed a high identity to plant FPP synthases. Expression of the gene in Escherichia coli yielded FPPS activity that catalyzed the synthesis of FPP as a main product. Tissue-specific and developmental analyses of the mRNA levels of CpFPPS and volatile sesquiterpenoids levels in C. praecox flowers revealed that the FPPS may play a regulatory role in floral volatile sesquiterpenoids of wintersweet.
- 相关文献
作者其他论文 更多>>
-
B and E MADS-box genes determine the perianth formation in Cymbidium goeringii Rchb.f
作者：Xiang, Lin;Fu, Xiaopeng;Zhao, Kaige;Zhang, Jie;Chen, Yue;Chen, Liping;Sun, Chongbo
关键词：
-
Genetic diversity, population structure, pollen morphology and cross-compatibility among Chinese Cymbidiums
作者：Li, Xiaobai;Xiang, Lin;Wang, Yan;Luo, Jie;Wu, Chao;Sun, Chongbo;Xie, Ming
关键词：Chinese Cymbidium;Zygopetalum;genetic diversity;population structure;pollen morphology;cross-compatibility
-
Deep Sequencing-Based Analysis of the Cymbidium ensifolium Floral Transcriptome
作者：Li, Xiaobai;Luo, Jie;Xiang, Lin;Qin, Dehui;Sun, Chongbo;Xie, Ming;Yan, Tianlian;Jin, Feng
关键词：
-
Functional analysis of FLOWERING LOCUS T orthologs from spring orchid (Cymbidium goeringii Rchb. f.) that regulates the vegetative to reproductive transition
作者：Xiang, Lin;Li, Xiaobai;Qin, Dehui;Guo, Fangqi;Wu, Chao;Miao, Lixiang;Sun, Chongbo
关键词：Flower induction;FLOWERING LOCUS T (FT);Gene expression;Spring orchid;Tobacco
