microRNA-8514-5p regulates adipokinetic hormone/corazonin-related peptide receptor to affect development and reproduction of Plutella xylostella
文献类型： 外文期刊
作者： Pang, Senbo 1 ; Wang, Shuo 1 ; Asad, Muhammad 1 ; Yu, Jiajing 1 ; Lin, Guifang 1 ; Chen, Jinzhi 6 ; Sun, Cuiying 1 ; Huang, Pengrong 1 ; Chang, Yanpeng 1 ; Wei, Hui 7 ; Yang, Guang 1 ;
作者机构： 1.Fujian Agr & Forestry Univ, Inst Appl Ecol, State Key Lab Ecol Pest Control Fujian & Taiwan Cr, Fuzhou, Peoples R China
2.Minist Educ, Joint Int Res Lab Ecol Pest Control, Fuzhou, Peoples R China
3.Fujian Agr & Forestry Univ, Ministerial & Prov Joint Innovat Ctr Safety Prod C, Fuzhou, Peoples R China
4.Minist Agr, Key Lab Integrated Pest Management Fujian Taiwan C, Fuzhou, Peoples R China
5.Fujian Prov Univ, Key Lab Green Pest Control, Fuzhou, Peoples R China
6.Wenzhou Acad Agr Sci, Wenzhou Vocat Coll Sci & Technol, Southern Zhejiang Key Lab Crop Breeding, Wenzhou, Zhejiang, Peoples R China
7.Fujian Acad Agr Sci, Inst Plant Protect, Fuzhou, Peoples R China
关键词： Dicer1; Plutella xylostella; miRNA; adipokinetic hormone/corazonin-related peptide receptor; development and reproduction
期刊名称：PEST MANAGEMENT SCIENCE （ 影响因子：3.8； 五年影响因子：4.3 ）
ISSN： 1526-498X
年卷期： 2024 年
页码：
收录情况： SCI
摘要： BACKGROUND: Dicer1 plays a crucial role in regulating the development and reproduction of insects. Knockout of Dicer1 causes pupal deformity, low eclosion and low fecundity in Plutella xylostella, but the mechanism behind this phenomenon is not clear. This study aims to identify differentially-expressed genes and miRNAs in the Dicer1-knockout strain (Delta PxDcr-1) and assess their impact on the reproduction and development of P. xylostella. RESULTS: The knockout of Dicer1 affected the expression of genes including the adipokinetic hormone/corazonin-related peptide receptor (PxACPR). The expression of PxACPR was upregulated, and the expression of miR-8514-5p was downregulated in Delta PxDcr-1 of P. xylostella. The dual luciferase reporter assay and pull-down assay showed that miR-8514-5p bound to PxACPR in vitro and in vivo. The expression profiles demonstrated a negative correlation between PxACPR mRNA and miR-8514-5p in different developmental stages of the wild-type strain. Both the miR-8514-5p agomir and double-stranded RNA of ACPR (dsPxACPR) injected into the pre-pupae inhibited the mRNA level of PxACPR, causing high mortality and deformity of pupae, and low fecundity and hatching rate, which were consistent with the phenotype of Delta PxDcr-1. The injection of miR-8514-5p antagomir caused a similar phenotype to the injection of miR-8514-5p agomir. Additionally, the injection of miR-8514-5p antagomir significantly rescued the phenotype caused by dsPxACPR. CONCLUSION: These results indicate that miR-8514-5p affects the development and reproduction of P. xylostella by regulating PxACPR, and the homeostasis of PxACPR expression is essential for the development and reproduction of P. xylostella. (c) 2024 Society of Chemical Industry.
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