文献类型： 外文期刊

作者： Dou, Mengying ¹ ; Zhang, Yao ² ; Shao, Qi ¹ ; Zhu, Jiali ¹ ; Li, Wang ¹ ; Wang, Xueying ¹ ; Zhang, Cai ¹ ; Li, Yuanxiao ¹ ;

作者机构： 1.Henan Univ Sci & Technol, Henan Int Joint Lab Anim Welf & Hlth Breeding, Luoyang, Peoples R China

2.Fujian Acad Agr Sci, Inst Agroecol, Fuzhou, Peoples R China

关键词： L-arginine; heat stress; bovine intestinal epithelial cells; antioxidant; inflammatory; apoptosis

期刊名称：ANIMAL BIOTECHNOLOGY （ 影响因子：2.141； 五年影响因子：1.92 ）

ISSN： 1049-5398

年卷期：

页码：

收录情况： SCI

摘要： Heat stress (HS) has a negative impact on the health and performance of dairy cows, resulting in economic losses. Damage to the intestinal epithelium is the main cause of the adverse effects of heat stress on bovine health. This study investigated the repair capability of L-arginine (L-Arg) in reducing the adverse effects of HS on bovine intestinal epithelial cells (BIECs). BIECs were treated as follows: (1) control cells were cultured at 37 degrees C continuously and received no L-Arg; (2) cells in HS group were grown at 42 degrees C for 6 h followed by 12 h at 37 degrees C; and (3) the L-Arg group was cultured at 42 degrees C for 6 h, then treated with L-Arg at 37 degrees C for 12 h. HS disrupted redox homeostasis and reduced viability in BIECs, while treatment with L-Arg (6 mmol/L) for 12 h markedly reduced the negative effects of HS. L-Arg protected cells by preventing HS-induced changes in mitochondrial membrane-potential, inflammation, apoptosis-related gene expression and regulation of antioxidant enzymes. The above results indicated that L-Arg reduced the level of damage from HS in BIECs by lowering oxidant stress and inflammation, suggesting that L-Arg could be an effective dietary addition to protect cows from adverse intestinal effects caused by HS.