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Overexpression of a GIPC glycosyltransferase gene, OsGMT1, suppresses plant immunity and delays heading time in rice

文献类型： 外文期刊

作者： Lin, Yuelong 1 ; Zhu, Yongsheng 2 ; Wang, Lanning 2 ; Zheng, Yanmei 2 ; Xie, Yunjie 2 ; Cai, Qiuhua 2 ; He, Wei 1 ; Xie, Hongguang 2 ; Liu, Haitao 2 ; Wang, Yingheng 1 ; Cui, Lili 2 ; Wei, Yidong 2 ; Xie, Huaan 1 ; Zhang, Jianfu 1 ;

作者机构： 1.Fujian Agr & Forestry Univ, Coll Agron, Fuzhou 350002, Peoples R China

2.Fujian Acad Agr Sci, Rice Res Inst, Fuzhou 350019, Peoples R China

3.Incubating Base State Key Lab Crop Germplasm Innov, State Key Lab Ecol Control Crop Pests Fujian & Tai, Natl Engn Lab Rice, South China Res Base State Key Lab Hybrid Rice, Fuzhou 350003, Fujian, Peoples R China

4.Minist Agr & Rural Areas South China, Fuzhou Branch, Natl Rice Improvement Ctr, Fujian Engn Lab Crop Mol Breeding,Fujian Key Lab R, Fuzhou 350003, Fujian, Peoples R China

关键词： GIPC; OsGMT1; Rice blast; MoNLP1; Calcein AM release assay; OsHAP5 gene family

期刊名称：PLANT SCIENCE （ 影响因子：5.2； 五年影响因子：5.7 ）

ISSN： 0168-9452

年卷期： 2023 年 331 卷

页码：

收录情况： SCI

摘要： Glycosylinositol phosphorylceramides (GIPCs) are the major sphingolipids in the plant plasma membrane. In Arabidopsis, mutations of genes involved in the synthesis of GIPCs affect many physiological aspects of plants, including growth, pollen fertility, defense, and stress signaling. Loss of function of the GIPC MANNOSYL-TRANSFERASE1 (AtGMT1) results in GIPC misglycosylation and induces plant immune responses accompa-nied by a severely dwarfed phenotype, thus indicating that GIPCs play important roles in plant immunity. Here, we investigated the enzymatic activity and phenotypes of transgenic lines of OsGMT1, the ortholog of AtGMT1. Sphingolipidomic analysis indicated that OsGMT1 retained the enzymatic activity of GIPC hexose (Hex) glyco-sylation, but the knockout lines did not accumulate H2O2. In contrast, the OsGMT1 overexpression lines showed significant down-regulation of several defense-associated or cell wall synthesis-associated genes, and enhanced sensitivity to rice blast. Furthermore, we first demonstrated the sensitivity of rice cells to MoNLP1 protein through calcein AM release assays using rice protoplasts, thus legitimizing the presence of MoNLPs in rice blast fungus. In addition, yeast two-hybrid screens using OsGMT1 as bait revealed that OsGMT1 may regulate heading time through the OsHAP5C signaling pathway. Together, our findings suggested clear physiological functional differentiation of GMT1 orthologs between rice and Arabidopsis.

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