The reference genome and full-length transcriptome of pakchoi provide insights into cuticle formation and heat adaption
文献类型： 外文期刊
作者： Xu, Huimin 1 ; Wang, Chunhua 1 ; Shao, Guirong 3 ; Wu, Shasha 1 ; Liu, Peng 1 ; Cao, Ping 3 ; Jiang, Peng 1 ; Wang, Shubin 1 ; Zhu, Hong 4 ; Lin, Xiao 3 ; Tauqeer, Arfa 1 ; Lin, Yizhang 3 ; Chen, Wei 4 ; Huang, Weiqun 4 ; Wen, Qingfang 5 ; Chang, Jiang 1 ; Zhong, Fenglin 1 ; Wu, Shuang 1 ;
作者机构： 1.Fujian Agr & Forestry Univ, Coll Life Sci, Fuzhou 350002, Peoples R China
2.Fujian Agr & Forestry Univ, Coll Hort, Fuzhou 350002, Peoples R China
3.Fujian Jinpin Agr Technol Co Ltd, Fuzhou 350000, Peoples R China
4.Fujian Seed Chief Stn, Fuzhou 350003, Peoples R China
5.Fujian Acad Agr Sci, Crop Res Inst, Fuzhou 350013, Peoples R China
期刊名称：HORTICULTURE RESEARCH （ 影响因子：7.291； 五年影响因子：7.487 ）
ISSN： 2662-6810
年卷期： 2022 年 9 卷
页码：
收录情况： SCI
摘要： Brassica rapa includes various vegetables with high economic value. Among them, green petiole type pakchoi (B. rapa ssp. chinensis) is one of the major vegetables grown in southern China. Compared with other B. rapa varieties, green petiole type pakchoi shows a higher level of heat resistance, which is partially derived from the rich epicuticular wax. Here we sequence a high-quality genome of green petiole type pakchoi, which has been widely used as the parent in breeding. Our results reveal that long terminal repeat retrotransposon insertion plays critical roles in promoting the genome expansion and transcriptional diversity of pakchoi genes through preferential insertions, particularly in cuticle biosynthetic genes. After whole-genome triplication, over-retained pakchoi genes escape stringent selection pressure, and among them a set of cuticle-related genes are retained. Using bulked-segregant analysis of a heat-resistant pakchoi cultivar, we identify a frame-shift deletion across the third exon and the subsequent intron of BrcCER1 in candidate regions. Using Nanopore long-read sequencing, we analyze the full-length transcriptome of two pakchoi cultivars with opposite sensitivity to high temperature. We find that the heat-resistant pakchoi cultivar can mitigate heat-caused leaf damage by activating an unfolded protein response, as well as by inhibiting chloroplast development and energy metabolism, which are presumably mediated by both transcriptional regulation and splicing factors. Our study provides valuable resources for Brassica functional genomics and breeding research, and deepens our understanding of plant stress resistance.
- 相关文献
作者其他论文 更多>>
-
Genome-Wide Identification and Expression Analyses of the Abscisic Acid Receptor PYR/PYL Gene Family in Response to Fruit Development and Exogenous Abscisic Acid in Luffa (Luffa cylindrica L.)
作者：Liu, Jianting;He, Shuilin;Liu, Jianting;Wang, Yuqian;Li, Zuliang;Wen, Qingfang;Zhu, Haisheng
关键词：
Luffa cylindrica ;PYR /PYL ; genome-wide identification; fruit development; expression analysis -
Deltacoronavirus HKU11, HKU13, PDCoV (HKU15) and HKU17 spike pseudoviruses enter avian DF-1 cells via clathrin-mediated endocytosis in a Rab5-, Rab7-and pH-dependent manner
作者：Liang, Qi-Zhang;Chen, Wei;Huang, Yu;Ji, Chun-Miao;Wang, Bin;Huang, Yao-Wei;Ji, Chun-Miao;Cong, Feng;Wang, Bin;Huang, Yao-Wei;Huang, Yao-Wei
关键词：Avian deltacoronavirus; porcine deltacoronavirus; entry pathway; pseudovirus; endocytosis
-
Genome-Wide Analyses and Expression Profiling of PME/PMEI Gene Families Reveal Their Relevance to Chilling Stress Response and Grafted Healing Efficiency in Cucumber/Pumpkin-Grafted Plants
作者：Lin, Huangfang;Ye, Huilan;Shao, Qingqing;Cheng, Saichuan;Bi, Ge;Lin, Biying;Lin, Honghui;He, Lei;Shen, Baoying;Liu, Shuang;Chen, Qingqing;Zhu, Haisheng;Wen, Qingfang
关键词：cucumber; pumpkin;
PME /PMEI gene family; graft healing; light intensity mode -
Genome-wide identification and characterization of lipoxygenases gene family in Luffa aegyptiaca revealed downregulation of LOX genes under heat stress
作者：Hao, Huang;Xue, Zhuzheng;Li, Yongping;Ma, Huifei;Wen, Qingfang;Zhu, Haisheng;Lin, Lianyu
关键词：Lipoxygenases;
Luffa aegyptiaca ; Tandem duplication; RNA-seq; Heat stress -
Transcriptomic and Metabolomic Joint Analysis Revealing Different Metabolic Pathways and Genes Dynamically Regulating Bitter Gourd (Momordica charantia L.) Fruit Growth and Development in Different Stages
作者：Qiu, Boyin;Li, Dazhong;Zhang, Qianrong;Lin, Hui;Li, Yongping;Wen, Qingfang;Zhu, Haisheng
关键词：bitter gourd (
Momordica charantia L.); fruit; growth and development; omics; gene expression -
Recombinase polymerase amplification combined with CRISPR/Cas12a technology for rapid on-site detection of duck adenovirus 3
作者：Liang, Qi-Zhang;Chen, Wei;Wang, Weiwei;Liu, Rong-Chang;Fu, Qiu-Ling;Fu, Guang-Hua;Cheng, Long-Fei;Jiang, Nan-Song;Chen, Hong-Mei;Huang, Yu;Chen, Wei;Zhu, Ting;Bi, Yuhai
关键词：DAdV-3; RPA; LFS; CRISPR/Cas12a; on-site detection
-
Development of a rapid on-site nucleic acid detection method for new genotype muscovy duck parvovirus based on RPA-CRISPR/Cas12a
作者：Liang, Qizhang;Chen, Wei;Wang, Weiwei;Liu, Rongchang;Fu, Qiuling;Fu, Guanghua;Cheng, Longfei;Jiang, Nansong;Chen, Hongmei;Huang, Yu
关键词：N-MDPV; RPA; LFS; CRISPR/Cas12a; on-site detection
