Fine-mapping and gene candidate analysis for AhRt1, a major dominant locus responsible for testa color in cultivated peanut
文献类型： 外文期刊
作者： Chen, Hao 1 ; Chen, Xiangyu 1 ; Xu, Rirong 1 ; Liu, Wenjing 3 ; Liu, Nian 2 ; Huang, Li 2 ; Luo, Huaiyong 2 ; Huai, Dongx 1 ;
作者机构： 1.Fujian Acad Agr Sci, Fujian Engn Res Ctr Characterist Upland Crops Bre, Inst Crop Sci,Minist Agr & Rural Affairs Peoples, Fujian Engn Lab Crop Mol Breeding,Fujian Res Stn, Fuzhou 350013, Peoples R China
2.Chinese Acad Agr Sci, Key Lab Biol & Genet Improvement Oil Crops, Minist Agr & Rural Affairs Peoples Republ China, Oil Crops Res Inst, Wuhan 430062, Peoples R China
3.Fujian Acad Agr Sci, Inst Qual Stand & Testing Technol Agroprod, Fujian Key Lab Agroprod Qual & Safety, Fuzhou 350003, Peoples R China
4.Quanzhou Inst Agr Sci, Jinjiang 362212, Peoples R China
期刊名称：THEORETICAL AND APPLIED GENETICS （ 影响因子：5.699； 五年影响因子：5.565 ）
ISSN： 0040-5752
年卷期： 2021 年 134 卷 11 期
页码：
收录情况： SCI
摘要： Key Message AhRt1 controlling red testa color in peanut was fine-mapped to an interval of 580 kb on chromosome A03, and one gene encoding bHLH transcriptional factor was identified as the putative candidate gene. Peanut with red testa has higher nutritional and economic value than the traditional pink testa varieties. Identification of genes controlling red testa color will accelerate the breeding program and facilitate uncovering the genetic mechanism. In this study, in order to identify gene underlying the red testa color in peanut, a F-2 population derived from a cross between a pink testa peanut variety "Fuhua 8" and a red testa variety "Quanhonghua 1" was constructed. The genetic analysis for the F-2 population revealed that the red testa color was controlled by one single dominant locus. This locus, named as AhRt1 (Arachis hypogaea Red Testa 1), was preliminary identified in chromosome A03 by BSA-sequencing analysis. Using a segregation mapping population, AhRt1 was fine-mapped to a 580-kb genomic region by substitution mapping strategy. Gene candidate analysis suggested that one predicted gene encoding bHLH transcriptional factor may be the possible candidate gene for AhRt1. A diagnostic marker closely linked to candidate gene has been developed for validating the fine-mapping result in different populations and peanut germplasm. Our findings will benefit the breeding program for developing new varieties with red testa color and laid foundation for map-based cloning gene responsible for red testa in peanut.
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