文献类型： 外文期刊
作者： Qin, QP 1 ; Zhang, SL 2 ; Chen, JW 2 ; Xie, M 2 ; Jin, YF 2 ; Chen, KS 2 ; Asghar, S 2 ;
作者机构： 1.Zhejiang Univ, Dept Hort, Hangzhou 310029, Peoples R China
2.Zhejiang Univ, Dept Hort, Hangzhou 310029, Peoples R China; Zhejiang Acad Agr Sci, Inst Hort, Hangzhou 310021, Peoples R China; Zhejiang Univ, Inst Biochem, Hangzhou 310029, Peoples R China
关键词： citrus;fructokinase;gene cloning;expression analysis;fructose accumulation
期刊名称：ACTA BOTANICA SINICA （ 影响因子：0.599； ）
ISSN：
年卷期：
页码：
收录情况： SCI
摘要： Fructokinase (FRK) is of primary importance in phosphorylation of fructose in plants. Two genomic DNA fragments encoding putative fructokinase gene were isolated from Citrus unshiu Marc, using PCR, which were named Cufrk1 and Cufrk2. A cDNA sequence same to the exons' sequences of Cufrk1 was amplified by RT-PCR and the full-length cDNA sequences of this gene was isolated using rapid amplification of cDNA ends (RACE) from mature fruit, named CuFRK1 (GenBank number: AY561840). Sequencing analysis showed that the amino acid sequences were 68% identical between Cufrk1 and Cufrk2. The full-length cDNA of CuFRK1 was 1 459 bp in length and contained a complete ORF from 168 to 1 220 bp, encoding 350 amino acids with a calculated molecular weight of 37.5 kD and a predicted pI of 5.03. The deduced amino acids of CuFRK1 possessed two sugar-binding domains, three ATP-binding domains and were 62% to 78% identical to the previously characterized fructokinase genes of other plants. Northern analysis showed that transcripts of CuFRK1 (Cufrk1) and Cufrk2 were detected at a high level in leaves, fruit at early developmental stages, but undetectable in peels and stems. Their expression patterns were distinct in petals and mature fruit. Enzymatic analysis showed that the activity of citrus fructokinase was decreasing with the fruit development, coincided with the accumulation of fructose in fruit. There was a significantly negative correlation between the decreasing fructokinase activity and the increasing fructose content during fruit development.
- 相关文献
作者其他论文 更多>>
-
Effect of preharvest Ca-chelate treatment on the storage quality of kiwifruit
作者：Xie, M;Jiang, GH;Zhang, HQ;Kawada, K
关键词：Ca concentration;flesh firmness;pectin substances;ethylene production;respiration rate;ascorbic acid;endogenous ABA level
-
Fruit Photosynthesis and Assimilate Translocation and Partitioning: Their Characteristics and Role in Sugar Accumulation in Developing Citrus unshiu Fruit
作者：Chen, JW;Zhang, SL;Zhang, LC;Zhao, ZZ;Xu, JG
关键词：satsuma mandarin;fruit photosynthesis;photosynthate;translocation;partitioning;sugar accumulation
