文献类型： 外文期刊

作者： Lin Xiaoyan ¹ ; Zhao Yuhua ¹ ; Fu Qinglin ³ ; Umashankara, M. L. ⁴ ; Feng Zhihong ⁵ ;

作者机构： 1.Zhejiang Univ, Coll Life Sci, Dept Microbiol, Hangzhou 310058, Zhejiang, Peoples R China

2.China Natl Rice Res Inct, Hangzhou 310006, Zhejiang, Peoples R China

3.Zhejiang Acad Agr Sci, Hangzhou 310021, Zhejiang, Peoples R China

4.Univ Mysore, Coll Yuvarajas, Dept Sericulture, Mysore 570005, Karnataka, India

5.Zhejiang Univ Technol, Zhijiang Coll, Hangzhou 310024, Zhejiang, Peoples R China

关键词： Bensulfuron-methyl (BSM);Microbial quantities;Community structures;DGGE

期刊名称：JOURNAL OF ENVIRONMENTAL SCIENCES （ 影响因子：5.565； 五年影响因子：5.066 ）

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收录情况： SCI

摘要： To investigate the influence of bensulfuron-methyl (BSM) on culturable microbial quantities and unculturable microbial community structures, conventional and molecular biological methods were employed in five BSM treated soils with three replications, respectively. The results obtained with traditional culture-dependent methods showed that a low-level of BSM had slight and transient effects on culturable microorganisms; nevertheless, high concentration of BSM resulted in a dramatic decrease in bacterial colony forming units (cfus). The result obtained using denaturing gradient gel electrophoresis (DGGE) revealed that more than 17 bands were observed in low BSM contaminated soil samples and only 10 bands were detected in samples with high BSM contamination. In other words, the diversity of soil community structure is related to the concentration of BSM. Cluster analysis showed that the community structure under low level of contamination was more similar to that of the control, while heavy contaminated amendments were far away from the above group. In a sense, the cooperation of the traditional method and the molecular biological method is more powerful to study the soil microbial information in contaminated ecosystem.