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Effects of calmodulin on DNA-binding activity of heat shock transcription factor in vitro

文献类型: 外文期刊

作者: Li, B 1 ; Liu, HT 2 ; Mu, RL 2 ; Sun, DY 2 ; Zhou, RG 2 ;

作者机构: 1.Hebei Normal Univ, Inst Mol Cell Biol, Shijiazhuang 050016, Peoples R China

2.Hebei Normal Univ, Inst Mol Cell Biol, Shijiazhuang 050016, Peoples R China; Hebei Acad Agr & Forestry Sci, Inst Agrophys Physiol & Biochem, Shijiazhuang 050051, Peoples R China

关键词: calmodulin;heat shock transcription factor;DNA-binding activity;maize;wheat;tomato

期刊名称:CHINESE SCIENCE BULLETIN ( 影响因子:1.649; 五年影响因子:1.738 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: The DNA-binding activity of heat shock transcription factor (HSF) was induced by heat shock (HS) of a whole cell extract. Addition of antiserum, specific to CaM, to a whole cell extract reduced bind of the HSF to the heat shock element (HSE) with maize, and the re-addition of CaM to the sample restored the activity of the HSF for binding to HSE. In addition, DNA-binding activity of the HSF was also induced by directly adding CaM to a whole cell extract at non-HS temperature with maize. Similar results were obtained with wheat and tomato. Our observations provide the first example of the involvement of CaM in regulation of the DNA-binding activity of the HSF.

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