文献类型： 外文期刊

作者： Chen, SB ¹ ; Liu, X ² ; Peng, HY ² ; Gong, WK ² ; Wang, R ² ; Wang, F ² ; Zhu, Z ² ;

作者机构： 1.Chinese Acad Sci, Inst Genet & Dev Biol, Beijing 100101, Peoples R China

2.Chinese Acad Sci, Inst Genet & Dev Biol, Beijing 100101, Peoples R China; Fujian Acad Agr Sci, Fujian Provincial Key Lab Agr Genet Engn, Fuzhou 350003, Peoples R China

关键词： cre/lox gene excision;transgenic rice;insect resistance;sck;cryIAc;marker-free

期刊名称：ACTA BOTANICA SINICA （ 影响因子：0.599； ）

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收录情况： SCI

摘要： Cre/lox-mediated gene excision in commercial rice (Oryza sativa L.) plants was studied with a recombination-reporter gene system, in which the selectable marker hygromycin phosphotransferase gene (hpt) flanking by two directly oriented lox sites was located between the rice actin1 promoter and a promoterless gusA gene. This system allows visualization of GUS expression by activating promoterless gusA after site-specific recombination. The crossing strategy was used to introduce the cre gene into the hx plants. In 30 hybrid plants from four crosses made from T_0 actin1 promoter-lox-hpt-lox -gusA plant with T_0 cre plant, 12 expressed GUS and 9 showed hygromycin-sensitive. We furthermore demonstrated the utility of the Cre/lox in excision of hpt marker gene in an elite indica rice restorer Minghui 86 transformed with both insecticidal modified cowpea trypsin inhibitor gene sck and Bacillus thuringi'ensis endotoxin gene cryIAc, In 77 hybrid plants from nine crosses made from T_2 homozygous lox-hpt-lox-sck-cryIAc plant with T_2 homozygous cre plant, 56 showed hygromycin-sensitive. Molecular analyses confirmed the excision of hpt in all hygromycin-sensitive plants.