您好，欢迎访问浙江省农业科学院 机构知识库！
浙江省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Medium Optimization for Enzymatic Production of L-Cysteine by Pseudomonas sp Zjwp-14 Using Response Surface Methodology

文献类型： 外文期刊

作者： Lv, Guo-Ying 1 ; Wang, Pu 1 ; He, Jun-Yao 1 ; Li, Xiao-Nian 3 ;

作者机构： 1.Zhejiang Univ Technol, Inst Biopharmaceut Engn, CN-310014 Hangzhou, Zhejiang, Peoples R China

2.Zhejiang Acad Agr Sci, CN-310021 Hangzhou, Zhejiang, Peoples R China

3.Zhejiang Univ Technol, Coll Chem Engn & Mat Sci, CN-310014 Hangzhou, Zhejiang, Peoples R China

关键词： medium optimization

期刊名称：FOOD TECHNOLOGY AND BIOTECHNOLOGY （ 影响因子：3.918； 五年影响因子：3.71 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Response surface methodology was applied to optimize medium constituents for enzymatic production of L-cysteine from DL-2-amino-Delta(2)-thiazoline-4-carboxylic acid (DL-ATC) by a novel Pseudomonas sp. Zjwp-14. With the Plackett-Burman design experiment, glycerol, DL-ATC, yeast extract, and pH were found to be the most powerful factors among the eight tested variables that influence intracellular enzyme activity for biotransformation of DL-ATC to L-cysteine. In order to investigate the quantitative effects for four variables selected from Plackett-Burman design on enzyme activity,) a central composite design was subsequently employed for further optimization. The determination coefficient (R-2) was 0.9817, and the results show that the regression models adequately explain the data variation and represent the actual relationships between the parameters and responses. The optimal medium for Pseudomonas sp. Zjwp-14 was composed of (in g/L): glycerol 16.94, DL-ATC 4.59, yeast extract 6.99, NaCl 5.0, peptone 5.0, beef extract 5.0, MgSO4 center dot 7H(2)O 0.4, and pH=7.94. Under the optimal conditions, the maximum intracellular enzyme activity of 918.7 U/mL in theory and 903.6 U/mL in the experiment were obtained, with an increase of 15.6 % compared to the original medium components. In a 5-litre fermentor, cultivation time for Pseudomonas sp. Zjwp-14 was cut down for 6 h and the maximum enzyme activity reached 929.6 U/mL.

  • 相关文献

[1]Production of Mycelial Biomass and Exo-polymer by Hericium erinaceus CZ-2: Optimization of Nutrients Levels using Response Surface Methodology. Cui, Fengjie,Zhang, Zhicai,Dong, Ying,Huang, Daming,Cui, Fengjie,Liu, Zhiqiang,Ping, Liying,Liu, Zhiqiang,Li, Yin,Ping, Lifeng.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：浙江省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台