文献类型： 外文期刊

作者： Zhang, Z. ¹ ; Zhao, L. ¹ ; Chen, X. ¹ ; Zheng, X. ¹ ;

作者机构： 1.CATAS, Inst Trop Biosci & Biotechnol, State Key Lab Trop Crop Biotechnol, Haikou 571101, Peoples R China

关键词： Methods and Techniques;Biochemistry and Molecular Biophysics

期刊名称：BIOLOGIA PLANTARUM （ 影响因子：1.747； 五年影响因子：2.146 ）

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收录情况： SCI

摘要： An efficient in vitro propagation of kava (Piper methysticum) was established. Utilizing 15-d-old tender shoots from dormant auxiliary buds as explants, significant induction of vigorous aseptic cluster shoots was achieved in Murashige and Skoog (MS) medium containing 0.5 mg dm-3 6-benzyladenine (BA), 0.5 mg dm(-3) indole-3-acetic acid (IAA), and antibiotics after 30 d. In vitro rooting was achieved at 100 % efficiency in MS medium containing 0.75 to 1.00 mg dm(-3) IAA or indole-3-butyric acid and 3 % sucrose. The most robust and long roots were observed in medium with IBA. Moreover, the embryonic callus was induced from petioles in MS medium supplemented with 1.0 mg dm(-3) BA and 0.1 mg dm(-3) IAA, of which 70 % differentiated into shoots in the presence of 1.0 mg dm(-3) BA and 0.5 mg dm(-3) IAA.