文献类型： 外文期刊

作者： Hong, K ¹ ; Ma, Y ² ; Li, MQ ² ;

作者机构： 1.Chinese Acad Trop Agr Sci, Natl Key Biotechnol Lab Trop Crops, Key Lab Plant Protect Res Inst, Haikou 571101, Hainan, Peoples R China

2.Chinese Acad Trop Agr Sci, Natl Key Biotechnol Lab Trop Crops, Key Lab Plant Protect Res Inst, Haikou 571101, Hainan, Peoples R China; S China Univ Trop Agr, Sch Engn, Danzhou 571737, Hainan, Peoples R China

关键词： phytase;cassava dregs;nitrogen source;thermostability

期刊名称：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY （ 影响因子：2.926； 五年影响因子：2.685 ）

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收录情况： SCI

摘要： Phytases produced by numerous microorganisms and plants degradE phytic acid that has chelated with metal ions in food and feed. It is importanj to study phytase for the role of metal ions in nutrition of animals and human~ as well as in the reduction of organic phosphate content of aqueous environ. ment. This article reports on solid-state fermentation of phytase from a ne~ substrate of cassava dregs. Large quantities of cassava dregs are produced ir tropical areas as a byproduct of cassava starch processing. Protein and inor. ganic salts were found to be low in cassava dregs. Cassava dregs could bE employed for phytase synthesis after the addition of a nitrogen source and mineral salts. Ammonium nitrate was the best nitrogen source among thE nitrogen sources investigated, including beef extract, yeast extract, urea ammonium nitrate, sodium nitrate, and ammonium sulfate. Sodium dodecyl sulfate promoted phytase production from cassava dregs. A maximum phytase yield of 6.73 U / g of dry mass was obtained. The obtained phytasE was stable at feed-processing temperature, since 70% of initial enzyme activ. ity was maintained after 30 min of treatment at 75°C.