文献类型： 外文期刊

作者： Cui, Hai Lan ² ; Zhang, Chao Xian ¹ ; Wei, Shou Hui ¹ ; Zhang, Hong Jun ³ ; Li, Xiang Ju ¹ ; Zhang, Yan Qiu ³ ; Wang, ¹ ;

作者机构： 1.Chinese Acad Agr Sci, Inst Plant Protect, Key Lab Weed & Rodent Biol & Management, Beijing 100193, Peoples R China

2.State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China

3.Minist Agr, Inst Control Agrochem, Beijing 100125, Peoples R China

4.Hebei Acad Agr & Forestry Sci, Inst Food & Oil, Shijiazhuang 050031, Peoples R China

关键词： tribenuron-methyl: 101200-48-0;pesticide;herbicide;acetolactate synthase: 9027-45-6;EC 2.2.1.6;serine;proline 197;leucine;herbicide resistance

期刊名称：WEED SCIENCE （ 影响因子：2.713； 五年影响因子：2.868 ）

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收录情况： SCI

摘要： The molecular basis of resistance to tribenuron-methyl, an acetolactate synthase (ALS)-inhibiting herbicide was investigated in four resistant (R) and three susceptible (S) flixweed populations. The resistance level in the R populations was assessed in whole-plant pot experiments in a greenhouse, and resistance indices ranged from 723 to 1422. The ALS genes of the three S populations and four R populations were cloned and sequenced, and the full coding sequence of the ALS gene of flixweed was 2,004 bp. The sequences of the ALS genes of the three S populations collected from Shaanxi, Gansu, and Tianjin were identical. Comparison of the ALS gene sequences of the S and R populations with Arabidopsis revealed that proline at position 197 of the ALS gene was substituted by leucine in R population SSX-2, by alanine in R population SSX-3, and by serine in R populations TJ-2 and GS-2. In another study of two R flixweed populations from Hebei and Shaanxi, resistance was also related to mutation at position 197 of the ALS gene. Both studies confirmed tribenuron-methyl resistance in flixweed in China, with the resistance mechanism being conferred by specific ALS point mutations at amino acid position 197.