Ectopic over-expression of two apple Flowering Locus T homologues, MdFT1 and MdFT2, reduces juvenile phase in Arabidopsis
文献类型： 外文期刊
作者： Li, W. M. 1 ; Tao, Y. 1 ; Yao, Y. X. 1 ; Hao, Y. J. 1 ; You, C. X. 1 ;
作者机构： 1.Shandong Agr Univ, State Key Lab Crop Biol, Tai An 271018, Shandong, Peoples R China
2.Shandong Agr Univ, Coll Hort Sci & Engn, Tai An 271018, Shandong, Peoples R China
3.Chinese Acad Trop Agr Sci, S Subtrop Crop Res Inst, Zhanjiang 524091, Peoples R China
关键词： complementary DNA: cDNA;amino acid sequence;flowering locus T-2 protein: FT2-protein;GenBank sequence data;flowering regulation
期刊名称：BIOLOGIA PLANTARUM （ 影响因子：1.747； 五年影响因子：2.146 ）
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收录情况： SCI
摘要： To get insight into mechanism by which apple tree (Malus domestica Borkh.) regulates flowering, two apple flowering locus T (FT) homologues, MdFT1 and MdFT2, were isolated from the leaf cDNAs of cultivar Gala. The open reading frames (ORFs) of two MdFTs encoded 174 amino acids. The deduced amino acid sequence of MdFT1 and MdFT2 showed 94.3 % similarity to each other, while 72.6 and 76.0 % to AtFT protein, respectively. Semi-quantitative RT-PCR indicated their specific expression in leaves. Visualization of MdFT2-GFP fusion protein demonstrated its localization on membrane. Ectopic overexpression of either MdFT1 or MdFT2 in Arabidopsis significantly induced early flowering by activating the downstream flowering-related genes.
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