您好，欢迎访问北京市农林科学院 机构知识库！
北京市农林科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Functional analysis of tomato LeEIL1 in an Arabidopsis ein2 mutant

文献类型： 外文期刊

作者： Pan, Yu 1 ; Chen, Guoping 1 ; Lu, Chungui 2 ; Chen, Xuqing 3 ; Hu, Zongli 1 ;

作者机构： 1.Chongqing Univ, Coll Bioengn, Chongqing 400044, Peoples R China

2.Univ Nottingham, Sch Biosci, Multidisciplinary Ctr Integrat Biol, Loughborough LE12 5RD, Leics, England

3.Beijing Agrobiotechnol Res Ctr, Beijing 100089, Peoples R China

关键词： ethylene: 74-85-1;signaling;ethylene-insensitive-3: EIN3;signal transduction pathway;florescence

期刊名称：ACTA PHYSIOLOGIAE PLANTARUM （ 影响因子：2.354； 五年影响因子：2.711 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： The ETHYLENE-INSENSITIVE3 (EIN3)/EIN3-Like (EIL) EIN3/EILs, novel nuclear proteins, are located at the downstream position of the ethylene signal transduction pathway. LeEIL1, which is expressed in fruit throughout ripening, is key transcription factor in the ethylene signaling pathway in tomato. To reveal its function, the LeEIL1 gene has been transformed into and expressed in the ein2 mutant of Arabidopsis. The expression levels of the transgene in the single copy line, LeEIL1-ein2-b, were higher than those in the multiple-copy line, LeEIL1-ein2-a. The ethylene-insensitive phenotype of the ein2 mutant plants has been partially recovered by expression of LeEIL1. The florescence of LeEIL1-ein2-a and LeEIL1-ein2-b exceeded that of the ein2 mutant but was still less than that of wild type of Arabidopsis. The expression of four pathology-related genes (AtPR3, 4, AtPDF1.2 and AtGST2) has been analyzed in LeEIL1 transgenic ein2 mutant plants. The expression of AtPR3 and AtPR4, which was reduced in the ein2 mutant, was enhanced in the two transgenic Arabidopsis plants. The expression of the AtPDF1.2 gene was unaffected in the two transgenic Arabidopsis lines, the ein2 mutant and wild-type Arabidopsis plants. In addition, the expression level of AtGST2 in transgenic Arabidopsis plants was lower even than that in ein2 mutant and wild-type Arabidopsis plants.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：北京市农林科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台