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Cordysobin, a novel alkaline serine protease with HIV-1 reverse transcriptase inhibitory activity from the medicinal mushroom Cordyceps sobolifera

文献类型： 外文期刊

作者： Wang, Shou-Xian 1 ; Liu, Yu 3 ; Zhang, Guo-Qing 4 ; Zhao, Shuang 3 ; Xu, Feng 3 ; Geng, Xiao-Li 3 ; Wang, He-Xiang 1 ;

作者机构： 1.China Agr Univ, State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China

2.China Agr Univ, Dept Microbiol, Beijing 100193, Peoples R China

3.Beijing Acad Agr & Forestry Sci, Inst Plant Protect & Environm Protect, Beijing 100097, Peoples R China

4.Beijing Agr Univ, Key Lab Urban Agr N, Minist Agr, Beijing 102206, Peoples R China

关键词： Alkaline serine protease;Fruiting bodies;Mushroom;Cordyceps sobolifera;Purification;H1V-1 reverse transcriptase

期刊名称：JOURNAL OF BIOSCIENCE AND BIOENGINEERING （ 影响因子：2.894； 五年影响因子：2.746 ）

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收录情况： SCI

摘要： A novel serine protease, designated as cordysobin, was purified from dried fruiting bodies of the mushroom Cordyceps sobolifera. The isolation procedure utilized ion exchange chromatography on DEAE-cellulose and SP-Sepharose followed by gel filtration on Superdex 75. The protease did not adsorb on DEAE-cellulose but bound to SP-Sepharose. In sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), the protease resolved as a single band with an apparent molecular mass of 31 kDa. Its optimal pH was 10.0, and the optimal temperature was 65°C. The protease displayed a Km value of 0.41 uM and 13.44 μM·min~(-1) using Suc-Leu-Leu-Val-Tyr-MCA as substrate at pH 10.0 and 37°C. Protease activity was enhanced by the Fe~(2+) ion at low concentration range of 1.25-10 mM and was strongly inhibited by Hg~(2+) up to 1.25 mM. The protease was strongly inhibited by chymostatin and phenylmethylsulfonyl fluoride (PMSF), suggesting that it is a serine protease. It manifested significant inhibitory activity toward HIV-1 reverse transcriptase (RT) with an IC_(50) value of 8.2x10~(-3) μM, which is the highest anti-HTV-1 RT activity of reported mushroom proteins.

  • 相关文献

[1]Purification and Characterization a Novel Alkaline Serine Protease from the Medicinal Mushroom Cordyceps sobolifera. Shou-Xian Wang,Guo-Qing Zhang,Shuang Zhao,Feng Xu,Lan-Qing Wang,Xiao-Li Geng,Li-Li Meng,Yu Liu,He-Xiang Wang. 2012

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