文献类型： 外文期刊
作者： Li, Yi 1 ; Qu, Jianjun 1 ; Yang, Xiaoming 2 ; An, Lizhe 3 ;
作者机构： 1.Chinese Acad Sci, Cold & Arid Reg Environm & Engn Res Inst, Dunhuang Gobi & Desert Ecol & Environm Res Stn, Lanzhou 730000, Gansu, Peoples R China
2.Gansu Acad Agr Sci, Lanzhou 730070, Gansu, Peoples R China
3.Lanzhou Univ, Sch Life Sci, Lanzhou 730000, Gansu, Peoples R China
关键词： Moisture content;Physiological indices;RAPD;Seed storage;Ultra-dry;Zygophyllum xanthoxylon
期刊名称：BOTANICAL STUDIES （ 影响因子：2.787； 五年影响因子：2.871 ）
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年卷期：
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收录情况： SCI
摘要： This research aimed to determine whether ultra-dry storage improves the longevity of Zygophyllum xanthoxylon seeds. Moisture content of Z. xanthoxylon seeds was dried to 4.81%, 3.81%, 2.41% and 1.99% (w.b.) in a desiccating container with silica gel,and stored at 45°C, 25°C and 15°C for 24 months. The data from 24 months showed that the optimum moisture content for storage varies with temperature. Our results found that optimum moisture can not be considered independently of temperature. After ultra-drying the seeds were accelerated aged (50°C, 1 month), some physiological indices were tested. The results indicated that Dehydrogenase, POD, SOD and CAT activities of the ultra-dry seeds were higher than those of the control seeds, while volatilealdehydes and malondialdehyde were lower than the control group. The results indicate that moisture content of seed was a key index for storage at ambient temperature (25 °C) and 3.81% seem to be the best moisture content for ultra-dry seeds in our research. RAPD markers were also used to evaluate the genetic fidelity of seeds, all RAPD profiles from ultra-dry seeds were monomorphic and similar to non-ultra-dry seeds, we conclude that variation is almost absent in ultra-dry storage. From these results,we suggest that seed moisture content less than 5% enhances longevity and ultra-dry could be an economical way for conservation of the plant genetic resource.
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