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Isolation and Characterization of a Temperature-Regulated Microsomal Oleate Desaturase Gene (CtFAD2-1) from Safflower (Carthamus tinctorius L.)

文献类型： 外文期刊

作者： Guan, Ling-Liang 1 ; Xu, Ying-Wen 1 ; Wang, Yuan-Biao 1 ; Chen, Li 1 ; Shao, Jin-feng 1 ; Wu, Wei 1 ;

作者机构： 1.Sichuan Agr Univ, Agron Coll, Yaan 625014, Peoples R China

2.Chinese Acad Trop Agr Sci, Trop Crops Genet Resources Inst, Haikou, Peoples R China

关键词： Carthamus tinctorius L.;Microsomal oleate desaturase (FAD2);Saccharomyces cerevisiae;Gene expression;Real-time PCR

期刊名称：PLANT MOLECULAR BIOLOGY REPORTER （ 影响因子：1.595； 五年影响因子：2.042 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： A cDNA sequence, designated CtFAD2-1 and putatively encoding a delta-12 fatty acid desaturase, was isolated from total RNA of immature normal-type safflower (Carthamus tinctorius L.) seeds using a PCR approach. The deduced amino acid sequence showed three histidine boxes characteristic of all membrane-bound desaturase and a C-terminal endoplasmic reticulum retention signal. Phylogenetic analysis indicated that CtFAD2-1 is grouped with other plant FAD2 sequences which are specifically or highly expressed in developing seeds. Functional expression of the corresponding CtFAD2-1 cDNA in yeast confirmed that it encodes a microsomal oleate desaturase, partially converting oleic fatty acid to linoleic fatty acid. Our transcriptional analysis suggested that CtFAD2-1 is constitutively expressed, with maximum transcript accumulation, in developing seeds. The sequence of this microsomal oleate desaturase gene was also obtained from high oleic safflower genotypes, CtFAD2-1'. The comparison between the coding regions of CtFAD2-1 and CtFAD2-1' revealed that a deletion of cytosine (C) exists at the position +603 bp of CtFAD2-1'. Analysis of transcript level showed that the expression of CtFAD2-1' in high oleic acid genotype is significantly lower than that in normal genotypes during seed development. These results suggested that the change of CtFAD2-1' gene sequence results in the deactivation and lower transcription of delta-12 fatty acid desaturase in high oleic safflower genotypes.

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