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Development and characterization of intron-flanking EST-PCR markers in rubber tree (Hevea brasiliensis Muell. Arg.)

文献类型： 外文期刊

作者： Li, Dejun 1 ; Xia, Zhihui 2 ; Deng, Zhi 1 ; Liu, Xianghong 1 ; Dong, Junmei 2 ; Feng, Fuying 3 ;

作者机构： 1.Chinese Acad Trop Agr Sci, State Key Lab Breeding Base Cultivat & Physiol Tr, Rubber Res Inst, Key Open Lab Rubber Biol,Minist Agr, Danzhou 571737, Hainan, Peoples R China

2.Hainan Univ, Coll Agr, Inst Biol Sci & Technol, Haikou 570228, Peoples R China

3.Inner Mongolia Agr Univ, Coll Life Sci, Hohhot 010018, Inner Mongolia, Peoples R China

关键词： Castor bean;Intron-flanking markers;Polymorphic information content;Rubber tree;Tapping panel dryness

期刊名称：MOLECULAR BIOTECHNOLOGY （ 影响因子：2.695； 五年影响因子：2.303 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： With a long-term goal of constructing a linkage map enriched with gene-specific markers in rubber tree (Hevea brasiliensis Muell. Arg.), we utilized rubber tree ESTs associated with tapping panel dryness (TPD) to develop intron-flanking PCR markers. After downloading and assembling the rubber tree ESTs associated with TPD, we predicted the exon/exon junction sites (E/E) by aligning rubber tree transcripts with the genomic sequences of castor bean (Ricinus communis L.). Based on the predicted E/E, the primers flanking intron(s) and no intron were designed. Compared with the markers designed by conventional method, the PCR success rate of the markers designed with the predicted E/E increased 28-30%, whereas the polymorphism rate of intron-flanking ESTPCR markers was approximately 3.43-fold increase. Therefore, the intron-flanking marker was more polymorphism- generating efficient than the markers designed by conventional methods. In addition, analyzing the polymorphic information content (PIC) among Hevea germplasm showed that the polymorphism of wild rubber tree accessions was higher than one of cultivated rubber tree clones and Hevea species. This study enriches the categories and numbers of molecular markers in rubber tree, and the markers developed in this research will have a wide application in DNA fingerprinting, marker-assisted selection and genetic mapping in rubber tree. This research also indicates that it is possible to develop intron-flanking ESTPCR markers of rubber tree with castor bean genome as reference sequences, which provides new insights into developing intron-flanking EST-PCR markers for rubber tree or other plant species without genomic information.

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