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Ultrasound-assisted extraction of polysaccharides from litchi (Litchi chinensis Sonn.) seed by response surface methodology and their structural characteristics

文献类型： 外文期刊

作者： Chen, Yeyuan 1 ; Luo, Haiyan 1 ; Gao, Aiping 1 ; Zhu, Min 1 ;

作者机构： 1.Chinese Acad Trop Agr Sci, Trop Crops Genet Resources Inst, Danzhou 571737, Peoples R China

关键词： Litchi seed;Polysaccharide;Structural identification;Ultrasound-assisted extraction

期刊名称：INNOVATIVE FOOD SCIENCE & EMERGING TECHNOLOGIES （ 影响因子：5.916； 五年影响因子：6.159 ）

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收录情况： SCI

摘要： Litchi seed has been applied in traditional Chinese medicine since ancient time. Its polysaccharides were prepared by ultrasound-assisted extraction technique and optimized by response surface methodology. The optimal conditions to obtain the highestyield of LSP were determined as follows: 15.0 ml/g of solvent/material ratio. 45.0 min of ultrasonic time and 222 W of ultrasonic power. By structural analysis, arabinose, fructose, galactose, glucose and mannose were detected both in LSPs I and II. A small amount of fucose was only found in LSP II. The monosaccharide composition indicated that LSPs I and II were neutral polysaccharides. The glycosidic linkages between monosaccharides and their relative molar percentages were also identified. Both LSPsI and II eliminated the DPPH radical in a dose-dependent manner. Furthermore, LSP I showed a better DPPH radical scavenging activity than LSP II. Industrial relevance: The bioactive polysaccharides, derived from plant sources, have attracted much attention by consumers and researchers due to their broad spectra of therapeutic properties and good safety. The structural characteristics of polysaccharides are highly correlated with their bioactivities. In this work, the extraction conditions of water-soluble polysaccharides from litchi seed were optimized by response surface methodology with the assistance of ultrasound. Their structural characteristics and bioactivity were investigated. The results are helpful to utilize litchi seed for food and biomedical application.

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