Identification of four novel alleles of the BoLA-DRB3 upstream regulatory region in Chinese yellow cattle
文献类型： 外文期刊
作者： Wang, K. 1 ; Sun, D. -X. 2 ; Li, K. -Y. 1 ; Wang, X. -Q. 1 ; Zhang, F. 1 ;
作者机构： 1.Hebei Acad Agr & Forestry Sci, Inst Cereal & Oil Crops, Shijiazhuang 050031, Peoples R China
2.China Agr Univ, Coll Anim Sci & Technol, Beijing 100093, Peoples R China
关键词： BoLA-DRB3 URR;DNA sequence;Polymorphism
期刊名称：TISSUE ANTIGENS （ 影响因子：1.348； 五年影响因子：1.508 ）
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收录情况： SCI
摘要： The sequence of upstream regulatory region (URR) of BoLA-DRB3 gene was amplified with polymerase chain reaction followed by DNA sequencing from six animals of Chinese yellow cattle. A total of five alleles including four newly identified ones, named BoLA-DRB3*R-03-U2, BoLA-DRB3*R-06-U2, BoLA-DRB3*R-07-U and BoLA-DRB3*R-12-U for the BoLA-DRB3 URR were found. Result of sequence analysis showed that the regulatory elements W, X, Y, CCAAT and TATA-like boxes existed in such URRs and 16 polymorphic sites (11 transitions, 3 transversions, 1 deletion and 1 insertion) located in the spacers between the conserved consensus boxes and 1 insertion within X box, while no new polymorphic site within the consensus boxes.
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