Uncovering small RNA-mediated responses to cold stress in a wheat thermosensitive genic male-sterile line by deep sequencing
文献类型： 外文期刊
作者： Tang, Zhonghui 1 ; Zhang, Liping 1 ; Xu, Chenguang 1 ; Yuan, Shaohua 1 ; Zhang, Fengting 1 ; Zheng, Yonglian 2 ; Zhao, 1 ;
作者机构： 1.Beijing Acad Agr & Forestry Sci, Beijing Engn & Tech Res Ctr Hybrid Wheat, Beijing 100097, Peoples R China
2.Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan 430070, Peoples R China
期刊名称：PLANT PHYSIOLOGY （ 影响因子：8.34； 五年影响因子：8.972 ）
ISSN：
年卷期：
页码：
收录情况： SCI
摘要： The male sterility of thermosensitive genic male sterile (TGMS) lines of wheat (Triticum aestivum) is strictly controlled by temperature. The early phase of anther development is especially susceptible to cold stress. MicroRNAs (miRNAs) play an important role in plant development and in responses to environmental stress. In this study, deep sequencing of small RNA (smRNA) libraries obtained from spike tissues of the TGMS line under cold and control conditions identified a total of 78 unique miRNA sequences from 30 families and trans-acting small interfering RNAs (tasiRNAs) derived from two TAS3 genes. To identify smRNA targets in the wheat TGMS line, we applied the degradome sequencing method, which globally and directly identifies the remnants of smRNA-directed target cleavage. We identified 26 targets of 16 miRNA families and three targets of tasiRNAs. Comparing smRNA sequencing data sets and TaqMan quantitative polymerase chain reaction results, we identified six miRNAs and one tasiRNA (tasiRNA-ARF [for Auxin-Responsive Factor]) as cold stress-responsive smRNAs in spike tissues of the TGMS line. We also determined the expression profiles of target genes that encode transcription factors in response to cold stress. Interestingly, the expression of cold stress-responsive smRNAs integrated in the auxin-signaling pathway and their target genes was largely noncorrelated. We investigated the tissue-specific expression of smRNAs using a tissue microarray approach. Our data indicated that miR167 and tasiRNA-ARF play roles in regulating the auxin-signaling pathway and possibly in the developmental response to cold stress. These data provide evidence that smRNA regulatory pathways are linked with male sterility in the TGMS line during cold stress.
- 相关文献
作者其他论文 更多>>
-
TaFAR9 and TaFAR10 synergistically regulate fertility conversion of photo-thermo-sensitive genic male sterility lines in wheat by modulating ROS homeostasis
作者：Niu, Fuqiang;Geng, Qiang;Hu, Lingling;Zhang, Li;Gao, Xiaoran;Liu, Jinke;Song, Xiyue;Liu, Zihan;Bai, Jianfang;Liu, Yongjie;Yuan, Shaohua;Zhai, Nuo;Zhao, Changping;Zhang, Liping
关键词：Photo-thermo-sensitive genic male sterility; Fatty acyl CoA reductase; Fertility conversion; Reactive oxygen species; Wheat
-
Analysis of Wheat Pollen Ole E I Proteins Reveals Potential Roles in Fertility and Stress Adaptation
作者：Zuo, Jinghong;Jia, Yanfeng;Wang, Weiwei;Guo, Chunman;Fang, Zhaofeng;Zhang, Yujuan;Fu, Jinzhou;Zhao, Sijia;Zhao, Changping;Wang, Dezhou;Yang, Guohang;Tang, Yimiao;Zuo, Jinghong;Wang, Weiwei;Guo, Chunman;Fang, Zhaofeng;Zhao, Changping;Wang, Dezhou;Tang, Yimiao;Jia, Yanfeng;Zhang, Yujuan;Fu, Jinzhou
关键词：abiotic stresses; anther development; expression pattern; Pollen
Olea europaea I (POEI); thermo-sensitive genic male sterile -
Transcriptomic and metabolomic analysis provides insights into lignin biosynthesis and accumulation and differences in lodging resistance in hybrid wheat
作者：Yang, Weibing;Zhang, Shengquan;Hou, Qiling;Gao, Jiangang;Wang, Hanxia;Chen, Xianchao;Liao, Xiangzheng;Zhang, Fengting;Zhao, Changping;Qin, Zhilie;Yang, Weibing;Zhang, Shengquan;Zhang, Fengting;Zhao, Changping
关键词：gene expression; lignin synthesis; lodging -resistance; hybrid wheat
-
Comparative transcriptome analysis reveals the impact of daily temperature difference on male sterility in photo-thermo-sensitive male sterile wheat
作者：Niu, Fuqiang;Song, Xiyue;Liu, Zihan;Liu, Yongjie;Bai, Jianfang;Zhang, Tianbao;Yuan, Shaohua;Bai, Xiucheng;Zhao, Changping;Zhang, Fengting;Sun, Hui;Zhang, Liping
关键词：Daily temperature difference; Male sterility; Safe seed production; Transcriptome; Wheat
-
Genome-Wide Identification and Expression Analysis of Sucrose Transporter Gene Family in Wheat Lines under Heat Stress
作者：Hou, Qiling;Gao, Jiangang;Qin, Zhilie;Sun, Hui;Wang, Hanxia;Yuan, Shaohua;Zhang, Fengting;Yang, Weibing
关键词：sucrose transporters; heat stress; gene expression; grain filling; male-sterile lines
-
Consensus linkage map construction and QTL mapping for eight yield-related traits in wheat using the BAAFS Wheat 90K SNP array
作者：Liu, Lihua;Qu, Pingping;Zhou, Yue;Li, Hongbo;Liu, Yangna;Zhang, Mingming;Zhang, Liping;Zhao, Changping;Zhang, Shengquan;Pang, Binshuang;Liu, Lihua;Qu, Pingping;Zhou, Yue;Li, Hongbo;Liu, Yangna;Zhang, Mingming;Zhang, Liping;Zhao, Changping;Zhang, Shengquan;Pang, Binshuang;Liu, Lihua;Qu, Pingping;Zhou, Yue;Li, Hongbo;Liu, Yangna;Zhang, Mingming;Zhang, Liping;Zhao, Changping;Zhang, Shengquan;Pang, Binshuang
关键词：consensus map; QTL; yield-related traits; wheat ( Triticum aestivum L.)
-
Physiological and Transcriptome Analyses Provide Insights into the Response of Grain Filling to High Temperature in Male-Sterile Wheat (Triticum aestivum L.) Lines
作者：Hou, Qiling;Gao, Jiangang;Wang, Hanxia;Qin, Zhilie;Sun, Hui;Yuan, Shaohua;Liang, Yulong;Wang, Changhua;Zhang, Fengting;Yang, Weibing
关键词：high temperature; gene expression; sucrose unloading; starch synthesis; grain filling
