文献类型： 外文期刊
作者： Deng, Liu-Hong 2 ; Luo, Ming-Wu 1 ; Zhang, Chun-Fa 2 ; Zeng, Hui-Cai 2 ;
作者机构： 1.Hainan Univ, Coll Mat Sci & Chem Engn, Haikou 570228, Peoples R China
2.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Haikou 571101, Peoples R China
关键词： rubber-tree leaves;RNA extraction;cetyltrimethylammonium bromide (CTAB);TRIzol;rapid amplification of cDNA ends (RACE)
期刊名称：BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY （ 影响因子：2.043； 五年影响因子：2.017 ）
ISSN：
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收录情况： SCI
摘要： A specific technique capable of producing high-quality RNA for rapid amplification of cDNA ends (RACE) was established for challenging tissues: leaves of the rubber tree. Total RNA was extracted by cetyltrimethylammonium bromide (CTAB)-LiCl combined with TRIzol reagent. The isolated RNA was highly intact. With RNA as template, full-length cDNA was obtained (NCBI, AY461413) by RACE.
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