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PhoR sequences as a phylogenetic marker to differentiate the species in the Bacillus subtilis group

文献类型： 外文期刊

作者： Guo, Qinggang 1 ; Li, Shezeng 1 ; Lu, Xiuyun 1 ; Li, Baoqing 1 ; Stummer, Belinda; Dong, Weixin 1 ; Ma, Ping 2 ;

作者机构： 1.Hebei Acad Agr & Forestry Sci, Inst Plant Protect, IPM Ctr Hebei Prov,Minist Agr, Key Lab Integrated Pest Management Crops No Reg N, Baoding 071000, Hebei, Peoples R China

2.Hebei Acad Agr & Forestry Sci, Inst Plant Protect, IPM Ctr Hebei Prov,Minist Agr, Key Lab Integrated Pest Management Crops No Reg N, Baoding 071000, Hebei, Peo

关键词： Bacillus;PhoR;Phylogeny;Species identification;Taxonomy

期刊名称：CANADIAN JOURNAL OF MICROBIOLOGY （ 影响因子：2.419； 五年影响因子：2.35 ）

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收录情况： SCI

摘要： Bacillus subtilis and its closely related species are indistinguishable from one another by morphological characteristics and 16S rDNA sequences. In this study, the partial phoR sequence was tested to determine the phylogenetic relationship of species in the B. subtilis group. Degenerate primers were developed according to the relatively conserved nucleotide sequences of phoR and the linked gene phoP in the B. subtilis group. The primers amplified a 1100 bp phoR fragment from strains representative of 6 species in the B. subtilis group. Based on the sequenced fragments, 26 type strains comprising these 6 species were clearly distinguished. At the intraspecies level, the phoR sequence similarities were 90%-100%, but at the interspecies level, the phoR sequence similarities were 32.8%-75%. Compared with the gyrB sequence, the phoR sequences showed a larger divergence especially at the interspecies levels. Therefore, the phoR sequence may be an efficient alternative marker for phylogenetic and taxonomic analysis of species in the B. subtilis group. Twenty-three Bacillus undomesticated isolates were tested for identification and phylogenetic analysis based on the phoR and gyrB sequences. The 23 isolates could be clearly delineated into 4 distinct groups, 10 as B. subtilis, 3 as B. mojavensis, 2 as B. atrophaeus, and 8 as B. amyloliquefaciens.

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