文献类型： 外文期刊

作者： Han, Miao ¹ ; Liu, Gang ¹ ; Li, Ji-Ping ² ; Govers, Francine ³ ; Zhu, Xiao-Qiong ¹ ; Shen, Chong-Yao ¹ ; Guo, Li-Yun ¹ ;

作者机构： 1.China Agr Univ, Dept Plant Pathol, Beijing 100193, Peoples R China

2.Gansu Acad Agr Sci, Inst Plant Protect, Lanzhou, Gansu, Peoples R China

3.Wageningen Univ, Phytopathol Lab, NL-6700 AP Wageningen, Netherlands

4.Ctr BioSyst Genom CBSG, Wageningen, Netherlands

关键词： Genotype;mating type;phenotype;potato late blight;SF-A2;SSR

期刊名称：JOURNAL OF EUKARYOTIC MICROBIOLOGY （ 影响因子：3.346； 五年影响因子：2.809 ）

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收录情况： SCI

摘要： The genetic diversity of 85 isolates of Phytophthora infestans collected in 2007 from Gansu province in China was determined and compared with 21 isolates collected before 2004. Among them, 70 belonged to the A1 mating type and 15 were self-fertile (SF). The mitochondrial DNA haplotypes revealed both Ia (25%) and IIa (75%) haplotypes. Metalaxyl resistance occurred with high frequency (54%) in Gansu. Simple sequence repeat (SSR) genotyping revealed 26 genotypes (13 from the Tianshui region) among the 85 isolates, and 18 genotypes among the 21 isolates collected before 2004, without overlap in genotypes detected in the two groups. Cluster analysis showed clear subdivisions within the different mating type isolates. Among Gansu's isolates, Nei's and Shannon's diversity indices were highest in isolates collected in Tianshui where both A1 and SF isolates were found. Analysis of molecular variance of isolates from Gansu indicated that 51% and 49% of the variance was explained by within-area and among-area variance, respectively. The results suggest that the occurrence of SF isolates increases the risk of sexual reproduction, the formation of oospore as initial inocula in the field, and affects the genotypic diversity in the population.