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First cloning and characterization of two functional aquaporin genes from an arbuscular mycorrhizal fungus Glomus intraradices

文献类型： 外文期刊

作者： Li, Tao 1 ; Hu, Ya-Jun 1 ; Hao, Zhi-Peng 1 ; Li, Hong 1 ; Wang, You-Shan 2 ; Chen, Bao-Dong 1 ;

作者机构： 1.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Urban & Reg Ecol, Beijing 100085, Peoples R China

2.Beijing Acad Agr & Forestry Sci, Inst Plant Nutr & Resources, Beijing 100097, Peoples R China

关键词： aquaporin;arbuscular mycorrhizal (AM);drought tolerance;Glomus intraradices;heterologous expression;osmotic stress

期刊名称：NEW PHYTOLOGIST （ 影响因子：10.151； 五年影响因子：10.475 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： Arbuscular mycorrhizal (AM) symbiosis is known to stimulate plant drought tolerance. However, the molecular basis for the direct involvement of AM fungi (AMF) in plant water relations has not been established. Two full-length aquaporin genes, namely GintAQPFI and GintAQPF2, were cloned by rapid amplification of cDNA 5'- and 3'-ends from an AMF, Glomus intraradices. Aquaporin localization, activities and water permeability were examined by heterologous expression in yeast. Gene expression during symbiosis was also analyzed by quantitative real-time polymerase chain reaction. GintAQPFI was localized to the plasma membrane of yeast, whereas GintAQPF2 was localized to both plasma and intracellular membranes. Transformed yeast cells exhibited a significant decrease in cell volume on hyperosmotic shock and faster protoplast bursting on hypo-osmotic shock. Polyethylene glycol (PEG) stimulated, but glycerol inhibited, the aquaporin activities. Furthermore, the expression of the two genes in arbuscule-enriched cortical cells and extraradical mycelia of maize roots was also enhanced significantly under drought stress. GintAQPFI and GintAQPF2 are the first two functional aquaporin genes from AMF reported to date. Our data strongly support potential water transport via AMF to host plants, which leads to a better understanding of the important role of AMF in plant drought tolerance.

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