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Isolation, characterization, and expression analysis of CmMLO2 in muskmelon

文献类型： 外文期刊

作者： Cheng, Hong 1 ; Kong, Weiping 1 ; Hou, Dong 1 ; Lv, Junfeng 2 ; Tao, Xinglin 1 ;

作者机构： 1.Gansu Acad Agr Sci, Inst Vegetable, Lanzhou 730070, Peoples R China

2.Gansu Agr Univ, Coll Vet Med, Lanzhou 730070, Peoples R China

关键词： Cloning;Expression;Melon;MLO

期刊名称：MOLECULAR BIOLOGY REPORTS （ 影响因子：2.316； 五年影响因子：2.357 ）

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收录情况： SCI

摘要： The full-length cDNA sequence of the MLO gene was cloned via SMART-RACE-PCR from muskmelon (Cucumis melo L.), and was designated as CmMLO2 (GenBank Accession No. FJ713542). The gene is 1,710 bp long and encodes a 570-amino acid peptide with a seven-transmembrane domain topology, and is a typical transmembrane protein. Localization analysis in onion epidermal cells showed that CmMLO2-GFP is localized in the plasma membrane. The expression of CmMLO2 gene was analyzed in melon leaf infected with powderymildew using a quantitative RT-PCR and it was found that CmMLO2 was mainly expressed in melon leaves in a no-tissue-specific pattern. Moreover, CmMLO2 may play a crucial role in the pathogenesis of powdery mildew.

  • 相关文献

[1]Molecular cloning and expression analysis of CmMlo1 in melon. Cheng, Hong,Kun, Weiping,Liu, Dongshun,Su, Yongquan,He, Qiwei.

[2]白粉病相关基因MLO在瓜菜类白粉病广谱抗性研究中的应用. 程鸿,孔维萍. 2015

[3]CmMLO2:一个与甜瓜白粉病感病相关的新基因. 程鸿,孔维萍,何启伟,王晓巍. 2013

[4]Effects of BTH and SA Treatment on Assimilate Allocation, Fruit Yield and Quality of Melon. Chen, Nianlai,Li, Xi-e,Hu, Ming,Zhang, Yuxin. 2010

[5]Isolation, chromosomal location, and expression analysis of putative powdery mildew resistance genes in wheat (Triticum aestivum L.). Wan, Ping,Ling, Lijun,Cao, Shuanghe,Wang, Xianping,Zhang, Wenjun,Ling, Hongqing,Zhu, Lihuang,Zhang, Xiangqi. 2007

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