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Metagenomic cloning and characterization of Na+/H+ antiporter genes taken from sediments in Chaerhan Salt Lake in China

文献类型： 外文期刊

作者： Wang, Xiaoyu 1 ; Xu, Feng 2 ; Chen, Sanfeng 1 ;

作者机构： 1.China Agr Univ, State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China

2.Beijing Acad Agr & Forestry Sci, Inst Plant & Environm Protect, Beijing 100097, Peoples R China

关键词： Antiport activity;Metagenomic library;Na+/H+ antiporter

期刊名称：BIOTECHNOLOGY LETTERS （ 影响因子：2.461； 五年影响因子：2.457 ）

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收录情况： SCI

摘要： A metagenomic library containing 8,000 clones was constructed by using genomic DNA obtained from Chaerhan Salt Lake in northwest China. Three Na+/H+ antiporters, C4-NhaG, C47-NhaG and C49-NhaG that grouped to the NhaG family, were screened and cloned from this metagenome by complementing Escherichia coli strain KNabc (Delta nhaA Delta nhaB Delta chaA) in medium containing 0.2 M NaCl. The three putative Na+/H+ antiporters were membrane proteins with 10, 11 and 11 transmembrane segments, respectively. They enabled E. coli KNabc to grow in medium containing 0.2-0.6 M Na+ or 7-14 mM Li+. Everted membrane vesicles prepared from E. coli KNabc cells carrying C49-NhaG exhibited Na+/H+ and Li+/H+ antiport activities.

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