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Selection of endogenous reference genes for gene expression analysis in the mediterranean species of the Bemisia tabaci (Hemiptera: Aleyrodidae) complex

文献类型： 外文期刊

作者： Su, Yun-Lin 1 ; He, Wen-Bo 1 ; Wang, Jia 2 ; Li, Jun-Min 3 ; Liu, Shu-Sheng 1 ; Wang, Xiao-Wei 1 ;

作者机构： 1.Zhejiang Univ, Inst Insect Sci, Minist Agr, Key Lab Agr Entomol, Hangzhou 310058, Zhejiang, Peoples R China

2.Southwest Univ, Coll Plant Protect, Chongqing Key Lab Entomol & Pest Control Engn, Chongqing 400716, Peoples R China

3.Zhejiang Acad Agr Sci, Inst Virol & Biotechnol, Hangzhou 310021, Zhejiang, Peoples R China

关键词： Bemisia tabaci;Endogenous reference gene;Gene expression;Quantitative PCR;RNA-seq

期刊名称：JOURNAL OF ECONOMIC ENTOMOLOGY （ 影响因子：2.381； 五年影响因子：2.568 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Quantitative real-time reverse transcription polymerase chain reaction is widely used for gene expression analysis, and robust normalization against stably expressed endogenous reference genes (ERGs) is necessary to obtain accurate results. In this study, the stability of nine housekeeping genes of the sweetpotato whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae) Mediterranean were evaluated in various conditions by quantitative real-time reverse transcription polymerase chain reaction using geNorm and Normfinder programs. Both programs suggested α-tubulin/ubiquitin and 18S small subunit ribosomal RNA the most stable genes for bacterium- and insecticide-treated whiteflies, respectively. For developmental stages, organs, and the samples including salivary glands and the whole body, transcription initiation factor TFIID subunit was calculated as the most stably expressed gene by both programs. In addition, we compared the RNA-seq data with the results of geNorm and Normfinder and found that the stable genes revealed by RNA-seq analysis were also the ERGs recommended by geNorm and Normfinder. Furthermore, the use of the most stable gene suggested by RNA-seq analysis as an ERG produced similar gene expression patterns compared with results generated from the normalization against the most stable gene selected by geNorm and Normfinder and multiple genes recommended by geNorm. It indicates that RNA-seq data are reliable and provide a great source for ERG candidate exploration. Our results benefit future research on gene expression profiles of whiteflies and possibly other organisms.

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