Development, characterization, genetic diversity and cross-species/genera transferability of ILP markers in rubber tree (Hevea brasiliensis)
文献类型: 外文期刊
作者: Li, Dejun 1 ; Xia, Zhihui 2 ; Deng, Zhi 1 ; Liu, Xianghong 2 ; Feng, Fuying 3 ;
作者机构: 1.Chinese Acad Trop Agr Sci, Key Open Lab Rubber Biol, State Key Lab Breeding Base Cultivat & Physiol Tr, Rubber Res Inst,Minist Agr, Danzhou 571737, Hainan, Peoples R China
2.Hainan Univ, Hainan Key Lab Sustainable Utilizat Trop Bioresou, Inst Biosci & Technol, Coll Agr, Haikou 570228, Peoples R China
3.Inner Mongolia Agr Univ, Coll Life Sci, Hohhot 010018, Inner Mongolia, Peoples R China
关键词: Rubber tree;Intron length polymorphism marker;Cross-species/genera transferability;Polymorphic information content;Tapping panel dryness
期刊名称:GENES & GENOMICS ( 影响因子:1.839; 五年影响因子:1.329 )
ISSN:
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收录情况: SCI
摘要: To construct a linkage map enriched with tapping panel dryness (TPD)-related markers, we firstly utilized rubber tree ESTs associated with TPD to develop intron length polymorphism (ILP) markers. In this study, 52 new ILP markers were further developed. Together with the ILP markers previously reported, 102 ILP markers developed from TPD-related ESTs were analyzed within 39 Hevea germplasm in detail. The PCR success rate and polymorphism rate of ILP markers was 97.06 and 61.62 %, respectively. The results based on PCR amplification and sequence analyses provided the evidences on cross-species/ genera transferability of rubber tree ILP markers. The average polymorphic information content (PIC) values of 39 Hevea germplasm were about 0.1719, indicating that the genetic base of Hevea germplasm selected in this study was very narrow. Among 39 Hevea germplasm, the PIC value of wild rubber tree accessions was the highest, followed by Hevea species and cultivated rubber tree clones. Based onthe similarity coefficient of ILP markers, 39 Hevea germplasm were divided into three groups including cultivated clones, wild accessions and Hevea species, suggesting that the classification was generally related to the characterization of Hevea germplasm. The ILP markers developed in this study further enriches the number of molecular marker in rubber tree, and the ILP markers will have a wide application in DNA fingerprinting, genetic diversity, marker-assisted selection and genetic mapping, etc. Moreover, the ILP markers transferred cross-euphorbiaceae family might be utilized in cassava, castor bean and physic nut.
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