Recovery of Native Protein from Potato Root Water by Expanded Bed Adsorption with Amberlite XAD7HP
文献类型： 外文期刊
作者： Zeng, Fan-Kui 1 ; Liu, Hong 2 ; Ma, Peng-Jun 1 ; Liu, Gang 1 ;
作者机构： 1.Chinese Acad Sci, Lanzhou Inst Chem Phys, Res & Dev Ctr Ecomat & Ecochem, Lanzhou 730000, Peoples R China
2.Chinese Acad Trop Agr Sci, Spice & Beverage Res Inst, Wanning 571533, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
关键词： potato root water;potato protein;protease inhibitors;expanded bed adsorption;Amberlite XAD7HP
期刊名称：BIOTECHNOLOGY AND BIOPROCESS ENGINEERING （ 影响因子：2.836； 五年影响因子：2.281 ）
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收录情况： SCI
摘要： Potato root water (PRW) contains ～1.5% protein. In this study, expanded bed adsorption (EBA) chromatography with Amberlite XAD7HP resin adsorbent was used to isolate native protein from crude PRW. The optimal pH and ionic strength for potato protein binding onto Amberlite XAD7HP were 5.0 and 20 mmol/L. The EBA-refined proteins were dried by vacuum freeze drying and spray drying at varying outlet temperatures. Results indicated that low temperature spray drying was the most cost effective method with respect to retaining protease inhibitor activities. The dried protein concentrates appeared bright yellow or dark reddish brown, with a total glycoalkaloid content of ～ 170 μg/g. The protease inhibitor activity was ～ 400 mg/g and 11～ 12 mg/g for trypsin inhibition and chymotrypsin inhibition, respectively. The results presented here suggest that EBA using Amberlite XAD7HP as the adsorbent is a feasible strategy for the direct adsorption of native protein from crude PRW.
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