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Proteome analysis of resistant and susceptible Cavendish banana roots following inoculation with Fusarium oxysporum f.sp cubense

文献类型： 外文期刊

作者： Lu, Ying 1 ; Liao, Dongjiang 2 ; Pu, Jinji 1 ; Qi, Yanxiang 1 ; Xie, Yixian 1 ;

作者机构： 1.Chinese Acad Trop Agr Sci, Environm & Plant Protect Inst, Hainan 571700, Peoples R China

2.Guangzhou Med Univ, Affiliated Hosp 1, Guangzhou Inst Resp Dis, State Key Lab Resp Dis, Guangzhou 510120, Peoples R China

关键词： Fusarium oxysporum f.sp. cubense;Comparative proteomic study;Susceptible and resistant Musa variety;Real time PCR

期刊名称：PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY （ 影响因子：2.747； 五年影响因子：2.388 ）

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收录情况： SCI

摘要： Fusarium wilt, caused by the soil-borne fungal pathogen Fusarium oxysporum f.sp. cubense tropical race 4 (TR 4), is regarded as a major yield-reducing factor in the banana industry worldwide. Not all the varieties of banana have the same level of resistance to TR 4, and understanding the molecular interactions in banana defense responses is an important tool to reveal the unexplained processes that underlie banana resistance to TR 4. A proteomic approach was used to explore how a susceptible variety (Williams) and a resistant variety (GCTCV-119) respond to TR 4 during infestation. Sixteen proteins spots were subjected to MALDI-TOF-MS. All of the proteins identified were related to metabolism, energy, immunity and defense, and unknown function. In the resistant variety, eight spots were more abundance, and six spots were less abundance relative to control. Of these, a total of five protein spots showed a-fold change in at least two time points. In the susceptible variety, five spots were more abundance and three spots were less abundance compared with the control. Of these, six spots changed significantly after challenge with TR 4. Chitinase, beta-1,3-glucanase and superoxide dismutase were further investigated using quantitative real time polymerase chain reaction (qRT-PCR) to determine whether the observed proteome changes are associated with changes in the mRNA levels or are due to post-transcriptional regulation. In the study, beta-1,3-glucanase and chitinase were found only in pathogen-challenged plants of the resistance variety compared with the control plants at 6, 12, 24, 48 and 72 hpi, but shown by qRT-PCR beta-1,3-glucanase and chitinase were expressed both in the resistance and susceptible variety in the roots of TR 4-infected bananas

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