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Efficient extraction technology of antioxidant crude polysaccharides from Ganoderma lucidum (Lingzhi), ultrasonic-circulating extraction integrating with superfine-pulverization

文献类型： 外文期刊

作者： Chen, Ti-Qiang 1 ; Wu, Yan-Bin 2 ; Wu, Jian-Guo 2 ; Ma, Lu 1 ; Dong, Zhi-Hai 3 ; Wu, Jin-Zhong 2 ;

作者机构： 1.Fujian Acad Agr Sci, Inst Edible & Med Fungi, Fuzhou 350013, Peoples R China

2.Fujian Univ Tradit Chinese Med, Acad Integrat Med, Fuzhou 350003, Peoples R China

3.HongXiangLong Biotechnol Developing Co Ltd, Beijing 10085, Peoples R China

关键词： Ganoderma lucidum;Crude polysaccharides;Antioxidant activity;Ultrasonic circulating extraction;Uniform design optimization

期刊名称：JOURNAL OF THE TAIWAN INSTITUTE OF CHEMICAL ENGINEERS （ 影响因子：5.876； 五年影响因子：5.294 ）

ISSN：

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收录情况： SCI

摘要： A novel and efficient extraction technique is proposed in this paper. Dried fruiting bodies of Ganoderma lucidum (Lingzhi) were finely pulverized into ultrafine powder, subsequent to extract the antioxidant crude polysaccharides by ultrasonic circulating extraction. Several important extraction factors were investigated by single factor analysis using DPPH· radical scavenging rate as an index. The extraction process was optimized by Uniform design using polysaccharide concentration as the index. Then the multiple regression equation was worked out, by which the optimal processing condition can be predicted, and the predicted model was experimentally verified. The highest polysaccharides concentration, i.e. 47.87 mg/ml, along with the highest DPPH· scavenging rate 53.63% can be achieved at 671 W ultrasonic power, 48 ℃ extraction temperature and 5.5/1 of intermit–running ratio (s/s), as well as 1:12.5 of solid–liquid ratio (w/v) and 45 min extraction time. The reproducible results confirmed that this novel technique was efficient for high extract yield of antioxidant crude polysaccharides from Lingzhi.

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