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Transcriptional mapping of the messenger and leader RNAs of orchid fleck virus, a bisegmented negative-strand RNA virus

文献类型： 外文期刊

作者： Kondo, Hideki 1 ; Maruyama, Kazuyuki 1 ; Chiba, Sotaro 1 ; Andika, Ida Bagus 2 ; Suzuki, Nobuhiro 1 ;

作者机构： 1.Okayama Univ, IPSR, Kurashiki, Okayama 7100046, Japan

2.Zhejiang Acad Agr Sci, Inst Virol & Biotechnol, Hangzhou 310021, Zhejiang, Peoples R China

关键词： Bipartite genome;Dichorhavirus;Gene junction;Leader RNA;Negative-strand RNA virus;Orchid fleck virus;Polyadenylated;RACE;Rhabdovirus;Transcription

期刊名称：VIROLOGY （ 影响因子：3.616； 五年影响因子：3.967 ）

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收录情况： SCI

摘要： The transcriptional strategy of orchid fleck virus (OFV), which has a two-segmented negative-strand RNA genome and resembles plant nucleorhabdoviruses, remains unexplored. In this study, the transcripts of six genes encoded by OFV RNA1 and RNA2 in the poly(A)-enriched RNA fraction from infected plants were molecularly characterized. All of the OFV mRNAs were initiated at a start sequence 3'-UU-5' with one to three non-viral adenine nucleotides which were added at the 5' end of each mRNA, whereas their 3' termini ended with a 5'-AUUUAAA(U/G)AAAA(A). n-3' sequence. We also identified the presence of polyadenylated short transcripts derived from the 3'-terminal leader regions of both genomic and antigenomic strands, providing the first example of plus- and minus-strand leader RNAs in a segmented minus-strand RNA virus. The similarity in the transcriptional strategy between this bipartite OFV and monopartite rhabdoviruses, especially nucleorhabdoviruses (family Rhabdoviridae) is additional support for their close relationship.

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