文献类型： 外文期刊

作者： Liu, Guo Xia ¹ ; Xuan, Ning ¹ ; Chu, Dong ¹ ; Xie, Hong Yan ¹ ; Fan, Zhong Xue ¹ ; Bi, Yu Ping ¹ ; Picimbon, Jean-Fran ¹ ;

作者机构： 1.Shandong Acad Agr Sci, Biotechnol Res Ctr, Shandong Prov Key Lab Genet Improvement Cultivat, Jinan 250100, Peoples R China

2.China Agr Univ, Dept Entomol, Beijing 100094, Peoples R China

3.Hebei Acad Agr & Forestry Sci,

关键词： whitefly;B-biotype;Q-biotype;chemosensory protein;neonicotinoid thiamethoxam insecticide

期刊名称：ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：1.698； 五年影响因子：1.758 ）

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收录情况： SCI

摘要： Chemosensory proteins (CSPs) are a group of small soluble proteins found so far exclusively in arthropod species. These proteins act in chemical communication and perception. In this study, a gene encoding the Type 1 CSP (BtabCSP1) from the agricultural pest Bemisia tabaci (whitefly) was analyzed to understand sequence variation and expression specificity in different biotypes. Sequence analysis of BtabCSP1 showed significant differences between the two genetically characterized biotypes, B and Q. The B-biotype had a larger number of BtabCSP1 mutations than the Q-biotype. Similar to most other CSPs, BtabCSP1 was more expressed in the head than in the rest of the body. One-step RT-PCR and qPCR analysis on total messenger RNA showed that biotype-Q had higher BtabCSP1 expression levels than biotype-B. Females from a mixed field-population had high levels of BtabCSP1 expression. The interaction of BtabCSP1 with the insecticide thiamethoxam was investigated by analyzing the BtabCSP1 expression levels following exposure to the neonicotinoid, thiamethoxam, in a time/dose-response study. Insecticide exposure increased BtabCSP1 expression (up to tenfold) at 4 and 24 h following 50 or 100 g/ml treatments.