您好，欢迎访问中国热带农业科学院 机构知识库！
中国热带农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Development of a real-time fluorescence loop-mediated isothermal amplification assay for rapid and quantitative detection of Fusarium mangiferae associated with mango malformation

文献类型： 外文期刊

作者： Pu, Jinji 1 ; Xie, Yixian 1 ; Zhang, He 1 ; Zhang, Xin 1 ; Qi, Yanxiang 1 ; Peng, Jun 1 ;

作者机构： 1.Chinese Acad Trop Agr Sci, Environm & Plant Protect Inst, Key Lab Integrated Pest Management Trop Crops, Minist Agr, Haikou 571101, Peoples R China

2.China Agr Univ, State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China

3.China Agr Univ, Minist Agr, Key Lab Plant Pathol, B

关键词： Fusarium mangiferae;Mango malformation disease;Real-time fluorescence loop-mediated isothermal amplification assay (RealAmp);Quantitative detection;Closed-tube visual detection

期刊名称：PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY （ 影响因子：2.747； 五年影响因子：2.388 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Fusarium mangiferae is a major causal agent of mango malformation disease (MMD) worldwide. Rapid and accurate detection of the causal pathogen is the cornerstone of integrated disease management. In this paper, a real-time fluorescence loop-mediated isothermal amplification assay (RealAmp) was developed for quantitative detection of E mangiferae in China. The LAMP primer set was designed based on a RAPD marker sequence and positive products were amplified only from F. mangiferae isolates, but not from any other species tested, showing a high specificity of the primer sets. The detection limit was approximately 2.26 x 10(-4) ng/mu l plasmid DNA when mixed with extracted mango DNA. Quantification of the pathogen DNA of MMD in naturally collected samples was no significant difference compared to classic real-time PCR Additionally, RealAmp assay was visual with an improved closed-tube visual detection system making the assay more convenient in diagnostics.

  • 相关文献

[1]Fusarium mangiferae对杧果酚类代谢的影响. 柳凤,欧雄常,詹儒林,韦继光,常金梅. 2015

[2]Fusarium mangiferae侵染对凯特杧内源激素含量的影响. 柳凤,欧雄常,詹儒林,韦继光,常金梅. 2015

[3]Fusarium mangiferae侵染对芒果矿质元素含量的影响. 柳凤,欧雄常,韦继光,詹儒林,常金梅. 2015

[4]海南省芒果畸形病的发现与鉴定. 张贺,韦运谢,漆艳香,刘晓妹,谢艺贤,喻群芳,蒲金基. 2015

[5]Transcription Profiling Analysis of Mango-Fusarium Mangiferae Interaction. Liu, Feng,Wu, Jing-bo,Zhan, Ru-lin,Ou, Xiong-chang. 2016

[6]Genetic diversity of Fusarium mangiferae isolated from mango malformation disease China. Liu, Feng,Wei, Ji-guang,Liu, Feng,Zhan, Ru-lin,Ou, Xiong-chang,Chang, Jin-mei. 2014

[7]Rapid and quantitative detection of citrus huanglongbing bacterium 'Candidatus Liberibacter asiaticus' by real-time fluorescent loop-mediated isothermal amplification assay in China. Wu, Xuhui,Meng, Chunliang,Wang, Guihua,Liu, Yuanzheng,Zhang, Xin,Yi, Kexian,Peng, Jun.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国热带农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台