Overexpression of CupB5 activates alginate overproduction in Pseudomonas aeruginosa by a novel AlgW-dependent mechanism
文献类型： 外文期刊
作者： de Regt, Anna K. 1 ; Yin, Yeshi 2 ; Withers, T. Ryan 2 ; Wang, Xin 4 ; Baker, Tania A. 1 ; Sauer, Robert T. 1 ; Yu, Ho 1 ;
作者机构： 1.MIT, Dept Biol, Cambridge, MA 02139 USA
2.Marshall Univ, Dept Biochem & Microbiol, Joan C Edwards Sch Med, Huntington, WV 25755 USA
3.Marshall Univ, Dept Pediat, Joan C Edwards Sch Med, Huntington, WV 25755 USA
4.Zhejiang Acad Agr Sci, Inst Plant Protect & Microbiol, Hangzhou 310021, Zhejiang, Peoples R China
5.Progenesis Technol LLC, Huntington, WV 25701 USA
期刊名称：MOLECULAR MICROBIOLOGY （ 影响因子：3.501； 五年影响因子：3.996 ）
ISSN：
年卷期：
页码：
收录情况： SCI
摘要： In Pseudomonas aeruginosa, alginate overproduction, also known as mucoidy, is negatively regulated by the transmembrane protein MucA, which sequesters the alternative sigma factor AlgU. MucA is degraded via a proteolysis pathway that frees AlgU from sequestration, activating alginate biosynthesis. Initiation of this pathway normally requires two signals: peptide sequences in unassembled outer-membrane proteins (OMPs) activate the AlgW protease, and unassembled lipopolysaccharides bind periplasmic MucB, releasing MucA and facilitating its proteolysis by activated AlgW. To search for novel alginate regulators, we screened a transposon library in the non-mucoid reference strain PAO1, and identified a mutant that confers mucoidy through overexpression of a protein encoded by the chaperone-usher pathway gene cupB5. CupB5-dependent mucoidy occurs through the AlgU pathway and can be reversed by overexpression of MucA or MucB. In the presence of activating OMP peptides, peptides corresponding to a region of CupB5 needed for mucoidy further stimulated AlgW cleavage of MucAin vitro. Moreover, the CupB5 peptide allowed OMP-activated AlgW cleavage of MucA in the presence of the MucB inhibitor. These results support a novel mechanism for conversion to mucoidy in which the proteolytic activity of AlgW and its ability to compete with MucB for MucA is mediated by independent peptide signals.
- 相关文献
作者其他论文 更多>>
-
Insect residual streams supplement improves chili pepper growth: Insights into the role of rhizosphere soil microbiome and metabolome
作者：Zhou, Hanghai;Zheng, Xusong;Shen, Qi;Yao, Xiaohong;Sun, Hong;Wang, Xin;Wu, Yifei;Tang, Jiangwu;Zhu, Zhigang;Yang, Chenghu;Li, Hua;Liu, Yuxue;Jiang, Lijia;Zhang, Chunfang
关键词：Insect farming; Insect residual stream; Chili pepper; Plant growth; Rhizosphere microbial community; Metabolic profile
-
Integrated Analysis of the Transcriptome and Metabolome Reveals the Network Regulating Fruit Taste in Sponge Gourd (Luffa cylindrica)
作者：Chai, Yaqian;Qiu, Wenjing;Gao, Luyao;Dong, Wenqi;Zhang, Peng;Zhou, Shengjun;Wang, Xin;Zhu, Yuqiang;Sun, Yuyan;Chai, Yaqian;Li, Zhikun
关键词：sponge gourd; taste; transcriptome; metabolome; mechanism
-
Enhancing Akkermansia growth via phytohormones: a strategy to modulate the gut-bone axis in postmenopausal osteoporosis therapy
作者：Zhao, Zhiqi;Zhu, Liying;Wang, Xin;Li, Xiaoqiong;Li, Jinjun;Zhao, Zhiqi;Zhu, Liying;Wang, Xin;Li, Xiaoqiong;Li, Jinjun;Zhao, Zhiqi;Sun, Haibiao;Han, Xiaoqiang;Deng, Yixuan;Li, Li
关键词：Osteoporosis; Phytohormones; Soybean isoflavone; Naringin; Denosumab; Gut microbiome
-
Raffinose-metabolizing bacteria impair radiation-associated hematopoietic recovery via the bile acid/FXR/NF-κB signaling pathway
作者：Jiao, Yang;Ren, Jiawei;Yuan, Nan;Shen, Jiaqi;Guo, Hongjuan;Cao, Jianping;Wu, Depei;Jiao, Yang;Ren, Jiawei;Yuan, Nan;Guo, Hongjuan;Cao, Jianping;Jiao, Yang;Wu, Depei;Qi, Xiaofei;Xie, Shichang;Zhou, Zhemin;Shen, Jiaqi;Wu, Depei;Qi, Xiaofei;Shen, Jiaqi;Wu, Depei;Qi, Xiaofei;Shen, Jiaqi;Wu, Depei;Yin, Huafang;Wang, Jian;Wang, Xin;Wang, Xin;Zhou, Zhemin;Zhou, Zhemin
关键词：Radiation-induced hematopoietic injury; gut microbiome;
Bacteroides acidifaciens ; raffinose-metabolizing bacteria; Intestinal-Hematopoietic Axis; UDCA; FXR -
Soil heterogeneity influences the biotoxicity of photoaged tire wear particles in Eisenia fetida: A comparative assessment
作者：Zhou, Hanghai;Wang, Xin;Sun, Hong;Li, Hua;Yao, Xiaohong;Wang, Yuan;Jin, Junwei;Tang, Jiangwu;Wu, Zhou;Li, Yongfu;Zhou, Jianjiang;Jiang, Lijia;Zhang, Chunfang
关键词：Tire wear particle; Photoaging; Earthworm; Soil heterogeneity; Oxidative damage
-
Regulatory Roles of Quercetin in Alleviating Fructose-Induced Hepatic Steatosis: Targeting Gut Microbiota and Inflammatory Metabolites
作者：Li, Jinjun;Li, Xiaoqiong;Li, Jinjun;Li, Xiaoqiong;Li, Jinjun;Zhao, Zhiqi;Li, Xinxin;Zhu, Liying;Wang, Xin;Li, Xiaoqiong;Li, Jinjun;Deng, Yixuan;Li, Li
关键词：fructose; gut microbiota; hepatic steatosis; quercetin
-
6PPD-quinone exposure induces oxidative damage and physiological disruption in Eisenia fetida: An integrated analysis of phenotypes, multi-omics, and intestinal microbiota
作者：Zhou, Hanghai;Wang, Xin;Sun, Hong;Li, Hua;Wang, Yuan;Yao, Xiaohong;Tang, Jiangwu;Wu, Zhou;Wu, Zhou;Jiang, Lijia;Zhang, Chunfang;Yan, Zhongyong
关键词：Earthworm; 6PPD-quinone; Oxidative stress; Multi-omics; Intestinal microbial community
