您好，欢迎访问浙江省农业科学院 机构知识库！
浙江省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Durable field resistance to wheat yellow mosaic virus in transgenic wheat containing the antisense virus polymerase gene

文献类型： 外文期刊

作者： Chen, Ming 1 ; Sun, Liying; Wu, Hongya 1 ; Chen, Jiong; Ma, Youzhi 1 ; Zhang, Xiaoxiang 1 ; Du, Lipu 1 ; Cheng, Shu 1 ;

作者机构： 1.Chinese Acad Agr Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Key Lab Biol & Genet Improvement Triticeae Crops, Inst Crop Sci,Minist Agr, Beijing 100193, Peoples R China

2.Chinese Acad Agr Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Key Lab Biol & Genet Improvement Triticea

关键词： transgenic wheat;wheat yellow mosaic virus;durable field resistance;RNA silencing;virus replicase gene

期刊名称：PLANT BIOTECHNOLOGY JOURNAL （ 影响因子：9.803； 五年影响因子：9.555 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Wheat yellow mosaic virus (WYMV) has spread rapidly and causes serious yield losses in the major wheat-growing areas in China. Because it is vectored by the fungus-like organism Polymyxa graminis that survives for long periods in soil, it is difficult to eliminate by conventional crop management or fungicides. There is also only limited resistance in commercial cultivars. In this research, fourteen independent transgenic events were obtained by co-transformation with the antisense NIb8 gene (the NIb replicase of WYMV) and a selectable gene bar. Four original transgenic lines (N12, N13, N14 and N15) and an offspring line (N12-1) showed high and durable resistance to WYMV in the field. Four resistant lines were shown to have segregated and only contain NIb8 (without bar) by PCR and herbicide resistance testing in the later generations. Line N12-1 showed broad-spectrum resistance to WYMV isolates from different sites in China. After growing in the infested soil, WYMV could not be detected by tissue printing and Western blot assays of transgenic wheat. The grain yield of transgenic wheat was about 10% greater than the wild-type susceptible control. Northern blot and small RNA deep sequencing analyses showed that there was no accumulation of small interfering RNAs targeting the NIb8 gene in transgenic wheat plants, suggesting that transgene RNA silencing, a common mechanism of virus-derived disease resistance, is not involved in the process of WYMV resistance. This durable and broad-spectrum resistance to WYMV in transgenic wheat will be useful for alleviating the damage caused by WYMV.

  • 相关文献

[1]Differential Characteristics of Viral siRNAs between Leaves and Roots of Wheat Plants Naturally Infected with Wheat Yellow Mosaic Virus, a Soil-Borne Virus. Li, Linying,Li, Linying,Xu, Yu,Zhang, Yan,Hu, Lifeng,Sun, Zongtao,Hong, Gaojie,Chen, Yang,Yan, Fei,Yang, Jian,Li, Junmin,Chen, Jianping,Li, Linying,Xu, Yu,Sun, Zongtao,Hong, Gaojie,Chen, Yang,Yan, Fei,Yang, Jian,Li, Junmin,Chen, Jianping,Andika, Ida Bagus,Xin, Xiangqi. 2017

[2]Sequence diversity in the coat protein coding region of wheat yellow mosaic bymovirus isolates from China. Chen, J,Chen, J,Du, J,Adams, MJ. 2000

[3]The P2 of Wheat yellow mosaic virus rearranges the endoplasmic reticulum and recruits other viral proteins into replication-associated inclusion bodies. Sun, Liying,Andika, Ida Bagus,Chen, Jianping,Shen, Jiangfeng,Yang, Di. 2014

[4]Silencing of NbXrn4 facilitates the systemic infection of Tobacco mosaic virus in Nicotiana benthamiana. Yan, Fei,Lu, Yuwen,Lin, Lin,Zheng, Hongying,Chen, Jianping,Yan, Fei,Lu, Yuwen,Lin, Lin,Zheng, Hongying,Chen, Jianping,Peng, Jiejun,Chen, Hairu,Yang, Jian,Jiang, Shanshan. 2011

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：浙江省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台