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Characterization of a new cry2Ab gene of Bacillus thuringiensis with high insecticidal activity against Plutella xylostella L.

文献类型： 外文期刊

作者： Pan, Zhizhen 1 ; Xu, Lian 2 ; Zhu, Yujing 1 ; Shi, Huai 1 ; Chen, Zheng 1 ; Chen, Meichun 1 ; Chen, Qingxi 2 ; Liu, Bo 1 ;

作者机构： 1.Fujian Acad Agr Sci, Agr Bioresources Inst, Fuzhou 350003, Peoples R China

2.Xiamen Univ, Sch Life Sci, State Key Lab Cellular Stress Biol, Xiamen 361005, Peoples R China

关键词： Bacillus thuringiensis;cry2Ab;Cloning;Homology modeling;Prokaryotic expression;Optimization;Insecticidal activity

期刊名称：WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY （ 影响因子：3.312； 五年影响因子：3.58 ）

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收录情况： SCI

摘要： Bacillus thuringiensis (Bt) strain FJAT-12 was a novel Bt strain isolated by Agricultural Bio-Resources Institute, Fujian Academy of Agricultural Science. In this study, a new cry2Ab gene was cloned from Bt strain FJAT-12 and named as cry2Ab30 by Bt delta-endotoxin Nomenclature Committee. The sequencing results showed there were two mutations in conservative sites which led to two amino acids modification. Homology modeling indicated that the two changes were located in beta-sheet of Domain II. A prokaryotic expression vector pET30a-cry2Ab30 was constructed and the expressed protein was analyzed by western blot using Cry2Ab antibody. The expression conditions including IPTG concentration, revolution and temperature were optimized to get the highest expression level by SDS-PAGE and BandScan. The bioassay results also showed that the Cry2Ab30 toxin had high insecticidal activity against Plutella xylostella and the LC50 value was 0.0103 mu g.mL(-1). The two mutations in beta-sheet of Domain II might contribute to insecticidal activity of Cry2Ab30 toxin against Plutella xylostella

  • 相关文献

[1]Proteolytic Activation of Bacillus thuringiensis Cry2Ab through a Belt-and-Braces Approach. Xu, Lian,Zhang, Jing,Chen, Qing-Xi,Pan, Zhi-Zhen,Liu, Bo,Zhu, Yu-Jing.

[2]Expression, purification and molecular characterization of a novel endoglucanase protein from Bacillus subtilis SB13. Guan, Xuefang,Chen, Penglian,Xu, Qingxian,Qian, Lei,Huang, Juqing,Lin, Bin.

[3]PxAPN5 serves as a functional receptor of Cry2Ab in Plutella xylostella (L.) and its binding domain analysis. Pan, Zhi-Zhen,Liu, Bo,Chen, Zheng,Zhu, Yu-Jing,Xu, Lian,Zhang, Jing,Chen, Qing-Xi.

[4]Insecticidal activity and antifeedant effect of a new type biocide GCSC-BtA against Plutella xylostella L. (Lep., Plutellidae). Sengonca, C,Liu, B,Zhu, YJ. 2006

[5]小菜蛾钙黏蛋白PxCR_(10-11)结构域的克隆、原核表达及功能分析. 许炼,刘波,潘志针,朱育菁,高焕娟,陈清西. 2017

[6]Molecular characterization and expression analysis of beta(2)-microglobulin in large yellow croaker Pseudosciaena crocea. Yu, Suhong,Chen, Xinhua,Ao, Jingqun,Yu, Suhong,Yu, Suhong. 2009

[7]Screening of Vip3Aa60 and Vip3Ad5 and characterization of their binding to Spodoptera exigua midguts. Pan, Zhi-Zhen,Guan, Xiong,Xu, Lian,Zhang, Jing,Chen, Qing-Xi,Gao, Huan-Juan,Pan, Zhi-Zhen,Liu, Bo,Chen, Zheng,Zhu, Yu-Jing. 2017

[8]Effect of chemical additives on Bacillus thuringiensis (Bacillales: Bacillaceae) against Plutella xylostella (Lepidoptera: Pyralidae). Zhang, L.,Huang, T.,Huang, Z.,Xu, L.,Wu, C.,Guan, X.,Qiu, S.,Gelbic, I..

[9]Effect of GCSC-BtA biocide on abundance and diversity of some cabbage pests as well as their natural enemies in southeastern China. Sengonca, C,Liu, B.

[10]Laboratory studies on the effect of the bioinsecticide GCSC-BtA on mortality and feeding of diamondback moth Plutella xylostella L. (Lepidoptera : Plutellidae) larvae on cabbage. Liu, B.,Zhu, Y. J.,Sengonca, C.. 2006

[11]Proteolytic activation of Bacillus thuringiensis Vip3Aa protein by Spodoptera exigua midgut protease. Zhang, Ling,Xu, Lian,Li, Jie,Niu, Li -Yang,Chen, Qing-Xi,Pan, Zhi-Zhen,Liu, Bo,Chen, Zheng,Zhu, Yu-Jing. 2018

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