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The length of an internal poly(A) tract of hibiscus latent Singapore virus is crucial for its replication

文献类型: 外文期刊

作者: Niu, Shengniao 1 ; Cao, Shishu 1 ; Huang, Li-Jing 1 ; Tan, Kelvin Chee-Leong 1 ; Wong, Sek-Man 1 ;

作者机构: 1.Natl Univ Singapore, Dept Biol Sci, Singapore 117543, Singapore

2.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Key Lab Trop Crop Biotechnol, Minist Agr, Haikou 571101, Hainan, Peoples R China

3.Temasek Life Sci Lab, Singapore 117604, Singapore

4.Natl Univ Singapore Suzhou Res Inst, Suzhou 215123, Jiangsu, Peoples R China

关键词: Tobamovirus;Internal poly(A) tract;Replication;CP expression;Poly(A) binding protein;RNA stability;RNA kissing structure

期刊名称:VIROLOGY ( 影响因子:3.616; 五年影响因子:3.967 )

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收录情况: SCI

摘要: Hibiscus latent Singapore virus (HLSV) mutants were constructed to study roles of its internal poly (A) tract (IPAT) in viral replication and coat protein (CP) expression. Shortening of the IPAT resulted in reduced HLSV RNA accumulation and its minimal length required for HLSV CP expression in plants was 24 nt. Disruption of a putative long range RNA-RNA interacting structure between 5' and 3' untranslated regions of HLSV-22A and -24A resulted in reduced viral RNA and undetectable CP accumulation in inoculated leaves. Replacement of the IPAT in HLSV with an upstream pseudoknot domain (UPD) of Tobacco mosaic virus (TMV) or insertion of the UPD to the immediate downstream of a 24 nt IPAT in HLSV resulted in drastically reduced viral RNA replication. Plants infected with a TMV mutant by replacement of the UPD with 43 nt IPAT exhibited milder mosaic symptoms without necrosis. We have proposed a model for HLSV replication. (C) 2014 Elsevier Inc. All rights reserved.

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