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Effects of PEGylated porcine glucagon-like peptide-2 therapy in weaning piglets challenged with lipopolysaccharide

文献类型： 外文期刊

作者： Qi, Ke-ke 1 ; Wu, Jie 1 ; Xu, Zi-wei 1 ;

作者机构： 1.Zhejiang Acad Agr Sci, Inst Anim Sci, Hangzhou 310021, Zhejiang, Peoples R China

关键词： PEGylated;Porcine glucagon-like peptide-2;Tight junction protein;Inflammatory cytokines;Caspase-3;Piglet

期刊名称：PEPTIDES （ 影响因子：3.75； 五年影响因子：3.389 ）

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收录情况： SCI

摘要： This study aims to evaluate the therapeutic effect of polyethylene glycosylated porcine glucagon-like peptide-2 (pGLP-2), a long-acting form of pGLP-2, in lipopolysaccharide (LPS)-challenged piglets. Eighteen 21-day-old weaning piglets were randomly assigned into three groups: control (saline solution), LPS (100 mug/kg LPS), and PEG-pGLP-2 (10 nmol/kg PEG-pGLP-2 +100 mug/kg LPS). All treatments were administered intraperitoneally. Compared with the control treatment, LPS treatment significantly decreased (P< 0.05) the villus heights of the duodenum and jejunum, as well as the villus height/crypt depth ratio of the jejunum. However, PEG-pGLP-2 therapy reduced these effects (P> 0.05). Specifically, PEG-pGLP-2 infusion significantly increased the villus height/crypt depth ratio of the duodenum (P< 0.05) compared with LPS treatment. Compared with the control treatment, LPS treatment significantly increased (P< 0.05) the mRNA expression levels of interferon-gamma (IFN-gamma) and tumor necrosis factor-alpha (TNF-alpha) in the jejunum. However, PEG-pGLP-2 therapy reduced these effects (P< 0.05). Specifically, PEG-pGLP-2 infusion significantly decreased (P< 0.05) the mRNA expression levels of interleukin (IL)-8 and TNF-a in the duodenum and jejunum, IL-10 in the duodenum, and IFN-gamma in the jejunum compared with the LPS treatment. LPS treatment increased the caspase-3 activity of the ileum mucosal (P<0.05), and this effect was significantly reduced by PEG-pGLP-2 treatment. These results indicate that PEG-pGLP-2 infusion alleviates the severity of intestinal injury in weaning piglets by reducing the secretion of inflammatory cytokines and the caspase-3 activity, and increasing the villus height/crypt depth ratio.

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[1]PEGylated porcine glucagon-like peptide-2 improved the intestinal digestive function and prevented inflammation of weaning piglets challenged with LPS. Qi, K. K.,Wu, J.,Deng, B.,Li, Y. M.,Xu, Z. W..

[2]Effect of porcine glucagon-like peptides-2 on tight junction in GLP-2R+IPEC-J2 cell through the PI(3)k/Akt/mTOR/p70(S6K) signalling pathway. Qi, K. K.,Sun, Y. Q.,Wan, J.,Deng, B.,Men, X. M.,Wu, J.,Xu, Z. W..

[3]兔出血症病毒在体外诱导细胞凋亡. 倪征,魏威,刘光清,陈柳,余斌,云涛,华炯钢,李双茂. 2009

[4]Therapeutic effects of different doses of polyethylene glycosylated porcine glucagon-like peptide-2 on ulcerative colitis in male rats. Qi, Ke-ke,Lv, Jia-jia,Wu, Jie,Xu, Zi-wei. 2017

[5]Porcine glucagon-like peptide-2 microspheres ameliorate inflammation in lipopolysaccharide-challenged weaning piglets. Wu, J.,Qi, K. K.,Xu, Z. W..

[6]Purified PEGylated porcine glucagon-like peptide-2 reduces the severity of colonic injury in a murine model of experimental colitis. Qi, Ke-ke,Wu, Jie,Wan, Jing,Men, Xiao-ming,Xu, Zi-wei.

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