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A recombinant field strain of Marek's disease (MD) virus with reticuloendotheliosis virus long terminal repeat insert lacking the meq gene as a vaccine against MD

文献类型： 外文期刊

作者： Su, Shuai 1 ; Cui, Ning 1 ; Zhou, Yu 2 ; Chen, Zimeng 1 ; Li, Yanpeng 3 ; Ding, Jiabo 4 ; Wang, Yixin 1 ; Duan, Luntao 1 ; C 1 ;

作者机构： 1.Shandong Agr Univ, Coll Vet Med, Tai An 271018, Shandong, Peoples R China

2.Beijing Acad Agr & Forestry Sci, Inst Anim Husb & Vet, Beijing 100097, Peoples R China

3.Chinese Acad Agr Sci, Biotechnol Res Inst, Beijing 100081, Peoples R China

4.China Inst Vet Drug Control, Beijing 100081, Peoples R China

关键词： Marek's disease virus;Recombinant MDV;REV LTR;Meq gene;Vaccine safety and efficacy;Cell culture passage

期刊名称：VACCINE （ 影响因子：3.641； 五年影响因子：3.816 ）

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收录情况： SCI

摘要： Marek's disease virus (MDV) GX0101, which is a field strain with a naturally occurring insertion of the reticuloendotheliosis virus (REV) long terminal repeat (LTR) fragment, shows distinct biological activities. Deletion of the meq gene in GX0101 contributes to its complete loss of pathogenicity and oncogenicity in SPF chickens, but this virus has a kanamycin resistance gene (kan(r)) residual at the site of meq gene. In the present study, the kanr was knocked out and a meq-null virus with a good replicative ability termed SC9-1 was selected. In vivo studies showed that SC9-1 had no pathogenicity or tumorigenicity to chickens. There were no obvious impacts on chicken weight, immune organ index or antibody levels induced by avian influenza virus (AIV)/newcastle disease virus (NDV) inactivated vaccines compared with the control group. The SC9-1 virus provided superior protection than CVI988/Rispens vaccine in both SPF chickens and Hy-line brown chickens when challenged with a very virulent MDV (rMd5 strain). There was no obvious change in SC9-1 protection against MDV rMd5 in SPF chickens after 20 passages in chicken embryonic fibroblast cells (CEFs). in conclusion, SC9-1 is a safe and effective vaccine candidate for the prevention of Marek's disease. (C) 2014 Elsevier Ltd. All rights reserved.

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