Cloning and characterization of GbGI, a DELLA-like gene from cotton (Gossypium barbadense)
文献类型: 外文期刊
作者: Yu, Xiaoling 1 ; Cui, Baiming 3 ; Ruan, Mengbin 1 ; Wen, Wei 1 ; Wang, Shuchang 1 ; Di, Rong 4 ; Peng, Ming 1 ;
作者机构: 1.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Haikou 571101, Hainan, Peoples R China
2.Hainan Univ, Coll Agr, Haikou 570228, Hainan, Peoples R China
3.Xinjiang Shihezi Univ, Coll Life Sci, Shihezi 832003, Xinjiang, Peoples R China
4.Rutgers State Univ, Dept Plant Biol & Pathol, New Brunswick, NJ 08901 USA
关键词: Cotton fiber;GA;DELLA protein;Transgene
期刊名称:PLANT GROWTH REGULATION ( 影响因子:3.412; 五年影响因子:3.691 )
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年卷期:
页码:
收录情况: SCI
摘要: The quality of cotton fiber is determined by the fiber development. Many factors affect fiber development of which hormones play an important role. Gibberellic acid (GA) is necessary for fiber elongation in cultured fertilized ovules of cotton (Gossypium barbadense), but the functional mechanism of GA on the promotion of fiber elongation is not completely clear. Our study indicated that the initiation and elongation of fiber are mediated by the application of GA to cultured ovules. Plant responses to GA are mediated by DELLA proteins. We isolated one DELLA-like protein gene, designated GbGI from G. barbadense. GbGI has all the typical motifs of DELLA proteins. Over-expression of GbGI cDNA in Arabidopsis plants resulted in the dwarf phenotype, indicating that GbGI functions as a GA suppressor. Real time RT-PCR analysis showed that GbGI was predominantly expressed in cotton fibers at late developing stage, indicating an important role that GbGI plays in cotton fiber elongation.
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