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Complete genomic sequence of a Pineapple mealybug wilt-associated virus-1 from Hainan Island, China

文献类型： 外文期刊

作者： Yu, Naitong 1 ; Luo, Zhiwen 1 ; Fan, Hongyan 1 ; Zhang, Zhili 1 ; Li, Xianghong 1 ; Wang, Jianhua 2 ; Liu, Zhixin 3 ; He, 1 ;

作者机构： 1.Minist Agr, Hainan Acad Agr Sci, Inst Trop Fruit Tree, Haikou Invest Stn Trop Fruit Trees, Hainanhaikou 571100, Peoples R China

2.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Haikou 571101, Hainan, Peoples R China

3.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechn

关键词： Genome;Pineapple mealybug wilt-associated virus-1;Sequencing

期刊名称：EUROPEAN JOURNAL OF PLANT PATHOLOGY （ 影响因子：1.907； 五年影响因子：2.022 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： This is the first report of the complete genome sequence of Pineapple mealybug wilt-associated virus-1 (PMWaV-1 HN, KJ872494) identified on pineapple [Ananas comosus (L.) Merr.] in Hainan island, China. The genome is 13,069 nucleotides long, possessing seven open reading frames (ORFs). The ORF1 encodes a 233 kDa polyprotein which has PRO protease domain, MTR methyltransferase domain and HEL helicase domain; The ORF2 similar to ORF7 encode RNA-dependent RNA polymerase protein (RdRp), a 6 kDa protein (p6), heat shock 70 homolog protein (HSP70h), a 46 kDa protein (p46), coat protein (CP) and a 24 kDa protein (p24), respectively. Full-length nucleotide sequence analysis of PMWaV-1 HN genome confirmed it 97.99 % homology with PMWaV-1 (AF414119) which was isolated from Hawaii, USA. Furthermore, based on Maximum Likelihood method, all conserved trees of RdRp, HSP70h and CP proteins sequences revealed that PMWaV-1 HN together with other PMWaV-1 isolates clearly separated into PMWaV-1 group.

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