The effects of modified atmosphere packaging on core browning and the expression patterns of PPO and PAL genes in 'Yali' pears during cold storage
文献类型： 外文期刊
作者： Cheng, Yudou 1 ; Liu, Liqin 1 ; Zhao, Guoqun 3 ; Shen, Chengguo 1 ; Yan, Hongbo 1 ; Guan, Junfeng 1 ; Yang, Kun 1 ;
作者机构： 1.Hebei Acad Agr & Forestry Sci, Inst Genet & Physiol, Shijiazhuang 050051, Peoples R China
2.Plant Genet Engn Ctr Hebei Prov, Shijiazhuang 050051, Peoples R China
3.Hebei Univ Sci & Technol, Coll Biosci & Bioengn, Shi
关键词： MAP;Core browning;PAL;PPO
期刊名称：LWT-FOOD SCIENCE AND TECHNOLOGY （ 影响因子：4.952； 五年影响因子：5.383 ）
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收录情况： SCI
摘要： Pear fruits often experience core browning when exposed to excessive CO2 during storage. Therefore, it is critical to understand the mechanism of this process to optimize atmospheric conditions during post-harvest pear storage. In the present study, the browning process, phenolic content, polyphenol oxidase (PPO) activity and expression profiles of phenylalanine ammonia-lyase (PAL) and PPO genes in the core tissue of 'Yali' pears (Pyrus bretschneideri Rehd cv. Yali) were investigated under modified atmosphere packaging (MAP). The results showed that MAP1 (with a thickness of 10 mu m) reduced core browning, retarded the peak appearance of PPO activity and phenolic content, and inhibited the expression of PbPAL1, PbPAL2, and PbPPO1 genes in core tissue relative to the control (which had no packaging), but MAP2 (with a thickness of 30 mu m) exerted the opposite effects during cold storage (0 degrees C). There was no significant relation between PbPPO4, PbPPO5 and PbPPO6 expression in core tissue and core browning. These results suggested that MAP1 was suitable for cold storage and that the PbPAL1, PbPAL2 and PbPPO1 genes might be involved in core browning under modified atmosphere storage in 'Yali' pears. (C) 2014 Elsevier Ltd. All rights reserved.
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